Centrisart Concentrator
0.5 - 2.5 ml samples
Centrisart I is a ready to use unit for small volume centrifugal ultrafiltration to separate proteins from low molecular weight substances in biological samples.
Centrisart I features a unique design, ultrafiltration in the opposite direction to the centrifugal force. This is so effective in preventing premature blockage of the filter that even whole blood samples can be deproteinized. The ultrafiltrate is collected in the floater insert tube, where it is readily accessible without removing the tube.
Typical applications include:
- Drug binding studies
- Determination of metabolites in serum
- Protein removal from blood samples
- Cleaning of liposomes Virus removal
|
Time to filter 50% of sample volume |
Time to filter 90% of sample volume |
Passage of sample species volume |
|
| BSA 1.0 mg / ml (66,000 MW) | |||
| 5,000 MWCO | 300 min | N / A | 0% |
| 10,000 MWCO | 35 min | 80 min | 2% |
| 20,000 MWCO | 9 min | 20 min | 2% |
| IgG 0.25 mg / ml (160,000 MW) | |||
| 100,000 MWCO | 13 min | 35 min | 3% |
| Blue Dextran 0.1 mg / ml (2,000,000 MW) | |||
| 300,000 MWCO | 9 min | 25 min | 28% |
* 2.5 ml samples were loaded into each device.
The devices were centrifuged at 2,000 g until the required filtrate volumes had been reached.
| Concentrator Capacity | |
| Swing Bucket Rotor | 2.5 ml |
| Fixed Angle Rotor | 2.5 ml |
| Dimensions | |
| Total Length | 93 mm |
| Width | 14 mm |
| Active Membrane Area | 0.79 cm² |
| Hold-up volume of membrane | < 5 µl |
| Dead stop volume | 100 µl |
| Materials of Construction | |
| Centrifuge tube | Polystyrene |
| Floater tube | Cellulose propionate |
| Cap | Polyethylene |
| Membrane | CTA, PES |
| Centrifuge | ||
| Rotor Type | Swing bucket | Fixed Angle |
| Minimum rotor angle | - | 25° |
| Rotor Cavity | To fit 15 ml (17 mm) conical bottom tubes | To fit 15 ml (17 mm) conical bottom tubes |
| Maximum Speed | 2,500 g | 2,000 g |
| Concentrate Recovery | ||
| Pipette type | Fixed or variable volume | Fixed or variable volume |
| Recommended tip | Thin gel loader type | Thin gel loader type |
How to Order
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