
PCR Grade Vivacons achieved by Ethylene Oxide Treatment
The new Vivacon 2 PCR Grade units, in addition to all features described below for Vivacon 2, have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 10-6 and free of detectable human genomic DNA contamination tested with a qPCR assay over 55 cycles with a verified limit of detection of < 2 pg.
Vivacon 2 are centrifugal concentrators for DNA concentration and buffer exchange applications with samples up to 2 ml. For optimal performance with very dilute samples, e.g. forensic specimens, Vivacon 2 is equipped with the patented Hydrosart® regenerated cellulose membrane that guarantees extremely low binding properties and high flux. High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-off printed on individual devices.
Vivacon 2 features a horizontal membrane design that is preferred by some users for reasons of consistency with previously used procedures and high recovery. The possibility of a back-spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.
qPCR assay over 55 cycles with a verified limit of detection of < 2 pg.
For smaller volumes of 500 µl (0.5 ml) and below Vivacon 500 ETO with identical features is available.
|
Sample size (bp) |
Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) |
|
| 2,000 MWCO | 10 | 120 min | 92 % | 7,500 |
| 10,000 MWCO | 30 | 60 min | 94 % | 5,000 |
| 30,000 MWCO | 50 | 60 min | 95 % | 2,500 |
| 50,000 MWCO | 300 | 45 min | 96 % | 2,500 |
| 100,000 MWCO | 600 | 30 min | 93 % | 2,500 |
Volume 2 ml, sample concentration 50 ng/ml Start volume: 2 ml
| Membrane | MWCO |
Double-Stranded Nucleotide Cut-off (bp) |
| Hydrosart® | 2 kDa | > 10 |
| Hydrosart® | 10 kDa | > 30 |
| Hydrosart® | 30 kDa | > 50 |
| Hydrosart® | 50 kDa | > 300 |
| Hydrosart® | 100 kDa | > 600 |
| Feature | Benefit |
| Re-spin possibility | Complete and highly reproducible sample recovery |
| Low binding material | High recoveries of low sample recovery |
| Easy to remove re-spin cap | Convenient sample handling |
| Graduation printed on | Optimal process control |
| Concentrator Capacity | |
| Fixed Angle Rotor | 2 ml |
| Dimensions | |
| Total Length | 125 mm (concentration) |
| Total Length | 115 mm (backspin) |
| Width | 16 mm |
| Active Membrane Area | 0.95 cm² |
| Hold-up volume membrane and support | 10 µl |
| Dead stop volume | 55 µl (25° rotor) |
| Materials of Construction | |
| Body and Filtrate Vessel | Polycarbonate |
| Back spin vial | Polypropylene |
| Concentrator Cap | Polypropylene |
| Membranes | Hydrosart® |
| Description | Prod. No. | Price | |
| PCR Grade, 30,000 MWCO, Hydrosart Membrane, 25 per pack | VN02H21ETO |
$110.00 |
Add to Cart |
| PCR Grade, 30,000 MWCO, Hydrosart Membrane, 100 per pack | VN02H22ETO |
$360.00 |
Add to Cart |
|
PCR Grade, 30,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN02H23ETO |
$1,600.00 |
Add to Cart |
| PCR Grade, 50,000 MWCO, Hydrosart Membrane, 25 per pack | VN02H31ETO |
$110.00 |
Add to Cart |
| PCR Grade, 50,000 MWCO, Hydrosart Membrane, 100 per pack | VN02H32ETO |
$360.00 |
Add to Cart |
|
PCR Grade, 50,000 MWCO, Hydrosart Membrane, 500 per pack For larger quantities - please contact us for a quotation. |
VN02H33ETO |
$1,600.00 |
Add to Cart |
| PCR Grade, 100,000 MWCO, Hydrosart Membrane, 25 per pack | VN02H41ETO |
$110.00 |
Add to Cart |
| PCR Grade, 100,000 MWCO, Hydrosart Membrane, 100 per pack | VN02H42ETO |
$360.00 |
Add to Cart |
|
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN02H43ETO |
$1,600.00 |
Add to Cart |