Vivacon 2

Vivacon 2

Reproducible DNA sample desalting and concentration

Vivacon 2 centrifugal concentrators offer the optimal solution for DNA concentration and buffer exchange applications. For optimal performance with very dilute samples, e. g. forensic samples, Vivacon 2 is equipped with the patented regenerated cellulose membrane Hydrosart®. High recoveries and excellent reproducibilities are paired with convenience offered by volume graduation and molecular weight cut-off printed on individual devices.

The possibility of a re-spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.

Vivacon 2 Applications

  • DNA rebuffering of organic extraction samples before sequencing reaction
  • Dye removal after sequencing reaction
  • Primer removal
  • Plasmid concentration
Performance Characteristics
Sample size
(bp)
Time to concentrate
up to 30x [min.]
at 20°C
Concentrate
recovery %
g-force
(xg)
2,000 MWCO 10 120 min 92 % 7,500
10,000 MWCO 30 60 min 94 % 5,000
30,000 MWCO 50 60 min 95 % 2,500
50,000 MWCO 300 45 min 96 % 2,500
100,000 MWCO 600 30 min 93 % 2,500

Volume 2 ml, sample concentration 50 ng/ml Start volume: 2 ml

Conversion Table for Hydrosart MWCO to Nucleotide Cut-off
Membrane MWCO Double-Stranded
Nucleotide Cut-off
(bp)
Hydrosart 2 kDa > 10
Hydrosart 10 kDa > 30
Hydrosart 30 kDa > 50
Hydrosart 50 kDa > 300
Hydrosart 100 kDa > 600
Feature Benefit
Re-spin possibility Complete and highly reproducible sample recovery
Low binding material High recoveries of low sample recovery
Easy to remove re-spin cap Convenient sample handling
Graduation printed on Optimal process control
Technical Specifications
Concentrator Capacity
Fixed Angle Rotor 2 ml
Dimensions
Total Length 125 mm (concentration)
Total Length 115 mm (backspin)
Width 16 mm
Active Membrane Area 0.95 cm²
Hold-up volume membrane and support 10 µl
Dead stop volume 55 µl (25° rotor)
Materials of Construction
Body and Filtrate Vessel Polycarbonate
Back spin vial Polypropylene
Concentrator Cap Polypropylene
Membranes Hydrosart®
Ordering Information
Description Prod. No. Price
2000 MWCO, Hydrosart Membrane, 25 per pack VN02H91 $100.00
Add to Cart
2000 MWCO, Hydrosart Membrane, 100 per pack VN02H92 $320.00
$300.00/each for 2
Add to Cart
2000 MWCO, Hydrosart Membrane, 500 per pack VN02H93 $1450.00
$1400.00/each for 2
$1275.00/each for 6
$1250.00/each for 10
Add to Cart
10,000 MWCO, Hydrosart Membrane, 25 per pack VN02H01 $100.00
Add to Cart
10,000 MWCO, Hydrosart Membrane, 100 per pack VN02H02 $320.00
$300.00/each for 2
Add to Cart
10,000 MWCO, Hydrosart Membrane, 500 per pack VN02H03 $1450.00
$1400.00/each for 2
$1275.00/each for 6
$1250.00/each for 10
Add to Cart
30,000 MWCO, Hydrosart Membrane, 25 per pack VN02H21 $100.00
Add to Cart
30,000 MWCO, Hydrosart Membrane, 100 per pack VN02H22 $320.00
$300.00/each for 2
Add to Cart
30,000 MWCO, Hydrosart Membrane, 500 per pack VN02H23 $1450.00
$1400.00/each for 2
$1275.00/each for 6
$1250.00/each for 10
Add to Cart
50,000 MWCO, Hydrosart Membrane, 25 per pack VN02H31 $100.00
Add to Cart
50,000 MWCO, Hydrosart Membrane, 100 per pack VN02H32 $320.00
$300.00/each for 2
Add to Cart
50,000 MWCO, Hydrosart Membrane, 500 per pack VN02H33 $1450.00
$1400.00/each for 2
$1275.00/each for 6
$1250.00/each for 10
Add to Cart
100,000 MWCO, Hydrosart Membrane, 25 per pack VN02H41 $100.00
Add to Cart
100,000 MWCO, Hydrosart Membrane, 100 per pack VN02H42 $320.00
$300.00/each for 2
Add to Cart
100,000 MWCO, Hydrosart Membrane, 500 per pack VN02H43 $1450.00
$1400.00/each for 2
$1275.00/each for 6
$1250.00/each for 10
Add to Cart
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