Vivacon 2
Reproducible DNA sample desalting and concentration
Vivacon 2 centrifugal concentrators offer the optimal solution for DNA concentration and buffer exchange applications. For optimal performance with very dilute samples, e. g. forensic samples, Vivacon 2 is equipped with the patented regenerated cellulose membrane Hydrosart®. High recoveries and excellent reproducibilities are paired with convenience offered by volume graduation and molecular weight cut-off printed on individual devices.
The possibility of a re-spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.
Vivacon 2 Applications
- DNA rebuffering of organic extraction samples before sequencing reaction.
- Dye removal after sequencing reaction.
- Primer removal.
- Plasmid concentration.
|
Sample size (bp) |
Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) |
|
| 2,000 MWCO | 10 | 120 min | 92 % | 7,500 |
| 10,000 MWCO | 30 | 60 min | 94 % | 5,000 |
| 30,000 MWCO | 50 | 60 min | 95 % | 2,500 |
| 50,000 MWCO | 300 | 45 min | 96 % | 2,500 |
| 100,000 MWCO | 600 | 30 min | 93 % | 2,500 |
Volume 2 ml, sample concentration 50 ng/ml Start volume: 2 ml
| Membrane | MWCO |
Double-Stranded Nucleotide Cut-off (bp) |
| Hydrosart® | 2 kDa | > 10 |
| Hydrosart® | 10 kDa | > 30 |
| Hydrosart® | 30 kDa | > 50 |
| Hydrosart® | 50 kDa | > 300 |
| Hydrosart® | 100 kDa | > 600 |
| Feature | Benefit |
| Re-spin possibility | Complete and highly reproducible sample recovery |
| Low binding material | High recoveries of low sample recovery |
| Easy to remove re-spin cap | Convenient sample handling |
| Graduation printed on | Optimal process control |
| Concentrator Capacity | |
| Fixed Angle Rotor | 2 ml |
| Dimensions | |
| Total Length | 125 mm (concentration) |
| Total Length | 115 mm (backspin) |
| Width | 16 mm |
| Active Membrane Area | 0.95 cm² |
| Hold-up volume membrane and support | 10 µl |
| Dead stop volume | 55 µl (25° rotor) |
| Materials of Construction | |
| Body and Filtrate Vessel | Polycarbonate |
| Back spin vial | Polypropylene |
| Concentrator Cap | Polypropylene |
| Membranes | Hydrosart® |
| Description | Prod. No. | Price | |
| 2,000 MWCO, Hydrosart Membrane, 25 per pack | VN02H91 |
$105.00 |
Add to Cart |
| 2,000 MWCO, Hydrosart Membrane, 100 per pack | VN02H92 |
$335.00 |
Add to Cart |
|
2,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN02H93 |
$1,525.00 |
Add to Cart |
| 10,000 MWCO, Hydrosart Membrane, 25 per pack | VN02H01 |
$105.00 |
Add to Cart |
| 10,000 MWCO, Hydrosart Membrane, 100 per pack | VN02H02 |
$335.00 |
Add to Cart |
|
10,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN02H03 |
$1,525.00 |
Add to Cart |
| 30,000 MWCO, Hydrosart Membrane, 25 per pack | VN02H21 |
$105.00 |
Add to Cart |
| 30,000 MWCO, Hydrosart Membrane, 100 per pack | VN02H22 |
$335.00 |
Add to Cart |
|
30,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN02H23 |
$1,525.00 |
Add to Cart |
| 50,000 MWCO, Hydrosart Membrane, 25 per pack | VN02H31 |
$105.00 |
Add to Cart |
| 50,000 MWCO, Hydrosart Membrane, 100 per pack | VN02H32 |
$335.00 |
Add to Cart |
|
50,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN02H33 |
$1,525.00 |
Add to Cart |
| 100,000 MWCO, Hydrosart Membrane, 25 per pack | VN02H41 |
$105.00 |
Add to Cart |
| 100,000 MWCO, Hydrosart Membrane, 100 per pack | VN02H42 |
$335.00 |
Add to Cart |
|
100,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN02H43 |
$1,525.00 |
Add to Cart |
How to Order
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