
PCR Grade Vivacons achieved by Ethylene Oxide Treatment
The new Vivacon 500 PCR Grade units, in addition to all features described for Vivacon 500, have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 10-6 and free of detectable human genomic DNA contamination tested by using a qPCR assay over 55 cycles with a verified limit of detection < 2 pg.
Vivacon 500 are centrifugal concentrators for the concentration of DNA or proteins and buffer exchange applications with samples up to 500 µl. For optimal performance with very dilute samples, e.g. forensic specimens, Vivacon 500 is equipped with the patented Hydrosart® regenerated cellulose membrane that guarantees extremely low binding properties and high flux. High recoveries and excellent reproducibility are paired with convenience offered by the molecular weight cut-off printed on individual devices.
Vivacon 500 features a horizontal membrane design that is preferred by some users for reasons of consistency with previously used procedures and high reproducibility. The possibility of a back-spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.
* qPCR assay over 55 cycles with a verified limit of detection < 2 pg.
For larger volumes up to 2 ml Vivacon 2 ETO with identical features is available.
|
Sample size (bp) |
Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) |
|
| 2,000 MWCO | 10 | 60 min | 93 % | 7,500 |
| 10,000 MWCO | 30 | 25 min | 94 % | 7,500 |
| 30,000 MWCO | 50 | 18 min | 88 % | 5,000 |
| 50,000 MWCO | 300 | 18 min | 91 % | 3,000 |
| 100,000 MWCO | 600 | 10 min | 87 % | 3,000 |
Start volume 0.5 ml, sample concentration 50 ng/ml
|
Sample size (bp) |
Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) |
|
| 2,000 MWCO | 0.25 mg/ml cytochrome c | 30 min | 95 % | 14,000 |
| 10,000 MWCO | 0.25 mg/ml cytochrome c | 15 min | 92 % | 14,000 |
| 30,000 MWCO | 1.0 mg/ml BSA | 10 min | 95 % | 14,000 |
| 50,000 MWCO | 1.0 mg/ml BSA | 10 min | 92 % | 14,000 |
| 100,000 MWCO | 1.0 mg/ml bovine lgG | 11 min | 90 % | 8,000 |
Start volume 0.5 ml, sample and concentration of protein as specified in table
| Membrane | MWCO |
Double-Stranded Nucleotide Cut-off (bp) |
| Hydrosart® | 2 kDa | > 10 |
| Hydrosart® | 10 kDa | > 30 |
| Hydrosart® | 30 kDa | > 50 |
| Hydrosart® | 50 kDa | > 300 |
| Hydrosart® | 100 kDa | > 600 |
| Feature | Benefit | Low binding material | High recoveries of low sample recovery |
| Re-spin possibility | Complete and highly reproducible sample recovery |
| Concentrator Capacity | |
| Fixed Angle Rotor | 0.5 ml |
| Dimensions | |
| Total Length | 45 mm (concentration) |
| Total Length | 47.5 mm (backspin) |
| Width | 12.4 mm |
| Active Membrane Area | 0.32 cm² |
| Hold-up volume membrane and support | < 5 µl |
| Dead stop volume | 5 µl (40° rotor) |
| Equipment Required Centrifuge | |
| Rotor type | Fixed angle |
| Minimum rotor angle | 40° |
| Rotor cavity | To fit 1.5 | 2.2 ml (11 mm) conical bottom tubes |
| Maximum speed | 14,000 g* |
| Materials of Construction | |
| Body | Polycarbonate |
| Filtrate Vessel | Polycarbonate |
| Membranes | Hydrosart® |
* Please note, Vivacon® 500 devices with membrane MWCO > 100 kDa need to be processed at lower g forces. See manuals for details.
| Description | Prod. No. | Price | |
| PCR Grade, 30,000 MWCO, Hydrosart Membrane, 25 per pack | VN01H21ETO |
$98.00 |
Add to Cart |
| PCR Grade, 30,000 MWCO, Hydrosart Membrane, 100 per pack | VN01H22ETO |
$325.00 |
Add to Cart |
|
PCR Grade, 30,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN01H23ETO |
$1,425.00 |
Add to Cart |
| PCR Grade, 50,000 MWCO, Hydrosart Membrane, 25 per pack | VN01H31ETO |
$98.00 |
Add to Cart |
| PCR Grade, 50,000 MWCO, Hydrosart Membrane, 100 per pack | VN01H32ETO |
$325.00 |
Add to Cart |
|
PCR Grade, 50,000 MWCO, Hydrosart Membrane, 500 per pack For larger quantities - please contact us for a quotation. |
VN01H33ETO |
$1,425.00 |
Add to Cart |
| PCR Grade, 100,000 MWCO, Hydrosart Membrane, 25 per pack | VN01H41ETO |
$98.00 |
Add to Cart |
| PCR Grade, 100,000 MWCO, Hydrosart Membrane, 100 per pack | VN01H42ETO |
$325.00 |
Add to Cart |
|
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN01H43ETO |
$1,425.00 |
Add to Cart |