Vivacon 500
Reproducible DNA sample desalting and concentration
Vivacon 500 centrifugal concentrators offer the optimal solution for DNA concentration and buffer exchange applications. For optimal performance with very dilute samples, e. g. forensic samples, Vivacon 500 is equipped with the patented regenerated cellulose membrane Hydrosart®. High recoveries and excellent reproducibilities are paired with convenience offered by volume graduation and molecular weight cut-off printed on individual devices.
Vivacon 500 can be used as well for concentration and buffer exchange of protein samples.
Vivacon 500 Applications
- DNA rebuffering of organic extraction samples before sequencing reaction
- Dye removal after sequencing reaction
- Primer removal
- Plasmid concentration
- Protein concentration
- Protein rebuffering
For larger volumes up to 2 ml Vivacon 2 with identical features is available.
Applications
- DNA rebuffering of organic extraction samples before sequencing reaction.
- Dye removal after sequencing reaction.
- Primer removal.
- Plasmid concentration.
|
Sample size (bp) |
Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) |
|
| 2,000 MWCO | 10 | 60 min | 93 % | 7,500 |
| 10,000 MWCO | 30 | 25 min | 94 % | 7,500 |
| 30,000 MWCO | 50 | 18 min | 88 % | 5,000 |
| 50,000 MWCO | 300 | 18 min | 91 % | 3,000 |
| 100,000 MWCO | 600 | 10 min | 87 % | 3,000 |
Start volume 0.5 ml, sample concentration 50 ng/ml
|
Sample size (bp) |
Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) |
|
| 2,000 MWCO | 0.25 mg/ml cytochrome c | 30 min | 95 % | 14,000 |
| 10,000 MWCO | 0.25 mg/ml cytochrome c | 15 min | 92 % | 14,000 |
| 30,000 MWCO | 1.0 mg/ml BSA | 10 min | 95 % | 14,000 |
| 50,000 MWCO | 1.0 mg/ml BSA | 10 min | 92 % | 14,000 |
| 100,000 MWCO | 1.0 mg/ml bovine lgG | 11 min | 90 % | 8,000 |
Start volume 0.5 ml, sample and concentration of protein as specified in table
| Membrane | MWCO |
Double-Stranded Nucleotide Cut-off (bp) |
| Hydrosart® | 2 kDa | > 10 |
| Hydrosart® | 10 kDa | > 30 |
| Hydrosart® | 30 kDa | > 50 |
| Hydrosart® | 50 kDa | > 300 |
| Hydrosart® | 100 kDa | > 600 |
| Feature | Benefit | Low binding material | High recoveries of low sample recovery |
| Re-spin possibility | Complete and highly reproducible sample recovery |
| Concentrator Capacity | |
| Fixed Angle Rotor | 0.5 ml |
| Dimensions | |
| Total Length | 45 mm (concentration) |
| Total Length | 47.5 mm (backspin) |
| Width | 12.4 mm |
| Active Membrane Area | 0.32 cm² |
| Hold-up volume membrane and support | < 5 µl |
| Dead stop volume | 5 µl (40° rotor) |
| Equipment Required Centrifuge | |
| Rotor type | Fixed angle |
| Minimum rotor angle | 40° |
| Rotor cavity | To fit 1.5 | 2.2 ml (11 mm) conical bottom tubes |
| Maximum speed | 14,000 g* |
| Materials of Construction | |
| Body | Polycarbonate |
| Filtrate Vessel | Polycarbonate |
| Membranes | Hydrosart® |
* Please note, Vivacon® 500 devices with membrane MWCO > 100 kDa need to be processed at lower g forces. See manuals for details.
| Description | Prod. No. | Price | |
| 2,000 MWCO, Hydrosart Membrane, 25 per pack | VN01H91 |
$94.00 |
Add to Cart |
| 2,000 MWCO, Hydrosart Membrane, 100 per pack | VN01H92 |
$312.00 |
Add to Cart |
| 10,000 MWCO, Hydrosart Membrane, 25 per pack | VN01H01 |
$94.00 |
Add to Cart |
| 10,000 MWCO, Hydrosart Membrane, 100 per pack | VN01H02 |
$312.00 |
Add to Cart |
| 30,000 MWCO, Hydrosart Membrane, 25 per pack | VN01H21 |
$94.00 |
Add to Cart |
| 30,000 MWCO, Hydrosart Membrane, 100 per pack | VN01H22 |
$312.00 |
Add to Cart |
|
30,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN01H23 |
$1,350.00 |
Add to Cart |
| 50,000 MWCO, Hydrosart Membrane, 25 per pack | VN01H31 |
$94.00 |
Add to Cart |
| 50,000 MWCO, Hydrosart Membrane, 100 per pack | VN01H32 |
$312.00 |
Add to Cart |
|
50,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN01H33 |
$1,350.00 |
Add to Cart |
| 100,000 MWCO, Hydrosart Membrane, 25 per pack | VN01H41 |
$94.00 |
Add to Cart |
| 100,000 MWCO, Hydrosart Membrane, 100 per pack | VN01H42 |
$312.00 |
Add to Cart |
|
100,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN01H43 |
$1,350.00 |
Add to Cart |