Vivaspin 15R Centrifugal Concentrator
2 - 15 ml samples
Vivaspin 15R is the latest member of the Vivaspin product family with all the unique features of Sartorius Stedim Biotech concentrators including a patented vertical membrane and a dead stop. Vivaspin 15R is targeting the volume segment 2 to 15 ml with a modified regenerated cellulose membrane; Hydrosart®. This membrane is ideal where extremely high recovery with very low adsorption is needed, for example in applications such as desalting and concentration of Ig fractions.
- Ultimate recovery at low adsorption (95-98%)
- Extremely short concentration time (30x in 15 min.)
- Convenient application protocol with easy handling
- Easy scale-up to Vivaflow 200 with Hydrosart® membrane for volumes up to 5 litres
- Very small hold up volume (< 20 µl)
Rotor Compatibility
Please note:
Vivaspin 15R (30 mm x 116 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus / Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes.
These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 Beckmann JA 20. If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the Vivaspin 15, which can be used in either round bottom or conical centrifuge tubes.
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Time to concentrate up to 30x [min.] at 20°C and solute recovery % |
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| Rotor | Swing bucket | 25 ° Fixed angle | ||
| Centrifugal force | 3,000 x g | 6,000 x g | ||
| Start volume | 15 ml | 12.5 ml | ||
| Min. | Rec. | Min. | Rec. | |
| Aprotonin 0.1 mg / ml* (6,500 MW) | ||||
| 5,000 MWCO | 47 | 95% | 45 | 95% |
| Cytochrome c 0.25 mg / ml* (12,400 MW) | ||||
| 5,000 MWCO | 45 | 96% | 45 | 96% |
| 10,000 MWCO | 25 | 94% | 18 | 94% |
| α-chymotrypsin 0.25 mg / ml* (25,000 MW) | ||||
| 5,000 MWCO | 50 | 98% | 45 | 98% |
| 10,000 MWCO | 25 | 98% | 18 | 98% |
| Ovalbumin 1.0 mg / ml* (45,000 MW) | ||||
| 10,000 MWCO | 20 | 98% | 14 | 98% |
| 30,000 MWCO | 15 | 94% | 12 | 94% |
| BSA 1.0 mg / ml* (66,000 MW) | ||||
| 30,000 MWCO | 18 | 98% | 15 | 98% |
| IgG 0.25 mg / ml* in DMEM (160,000 MW) | ||||
| 30,000 MWCO | 30 | 98% | 25 | 96% |
* proteins other than IgG made up in 50 mM potassium phosphate, 150 mM sodium chloride, pH 7.4
| Concentrator Capacity | |
| Swing bucket rotor | 15 ml |
| Fixed angle rotor | 12.5 ml |
| Dimensions | |
| Total Length | 116 mm |
| Width | 30 mm |
| Active Membrane Area | 3.9 cm² |
| Hold-up volume membrane | < 20 µl |
| Dead stop volume | 30 µl |
| Materials of Construction | |
| Body | Polycarbonate |
| Filtrate Vessel | Polypropylene |
| Concentrator Cap | Polycarbonate |
| Membrane | Hydrosart |
| Centrifuge | ||
| Rotor Type | Swing bucket | Fixed angle |
| Minimum rotor angle | - | 25° |
| Rotor Cavity | To fit 50 ml (30 mm) conical bottom tubes | To fit 50 ml (30 mm) conical bottom tubes |
| Maximum Speed | 3,000 g | 6,000 g |
| Concentrate Recovery | ||
| Pipette type | Fixed or variable volume | Fixed or variable volume |
| Recommended tip | Thin gel loader type | Thin gel loader type |