Vivaspin 500 Centrifugal Concentrator
100 µl to 500 µl samples
Vivaspin 500 µl centrifugal filter units offer a simple, one step procedure for sample preparation.
They can effectively be used in a fixed angle rotors accepting 2.2 ml centrifuge tubes.
The patented vertical membrane design and thin channel filtration chamber (US 5,647,990),
minimises membrane fouling and provides high speed concentrations, even with particle laden solutions.
Performance Characteristics
|
Time to concentrate
up to 30x [min.] at 20°C
|
Concentrate Recovery %
|
| Rotor |
fixed angle |
| Centrifugal force |
12,000 x g |
| Start volume |
500 µl |
| Aprotinin 0.25mg/ml (6,500 MW) |
| 3,000 MWCO PES |
30 |
96% |
| BSA 1.0 mg / ml (66,000 MW) |
| 5,000 MWCO PES |
15 |
96% |
| 10,000 MWCO PES |
5 |
96% |
| 30,000 MWCO PES |
5 |
95% |
| IgG 0.25 mg / ml (160,000 MW) |
| 30,000 MWCO PES |
10 |
96% |
| 50,000 MWCO PES |
10 |
96% |
| 100,000 MWCO PES |
10 |
96% |
Technical Specifications
| Concentrator Capacity |
| Swing Bucket Rotor |
do not use |
| Fixed Angle Rotor |
500 µl |
| Dimensions |
| Total Length |
50 mm |
| Width |
11 mm |
| Active Membrane Area |
0.5 cm² |
| Hold-up volume, membrane and support |
<5 µl |
| Dead stop volume |
5 µl |
| Materials of Construction |
| Body |
Polycarbonate |
| Filtrate Vessel |
Polypropylene |
| Concentrator Cap |
Polycarbonate |
| Membrane |
Polyethersulfone |
Equipment Required
| Centrifuge |
| Rotor Type |
Fixed angle |
| Minimum rotor angle |
40° |
| Rotor Cavity |
To fit 2.2 ml (11 mm) conical bottom tubes |
| Maximum Speed |
15,000 g |
| Concentrate Recovery |
| Pipette type |
Fixed or variable volume |
| Recommended tip |
Thin gel loader type |