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Proteus NoEndo™ spin columns provide fast convenient affinity-based removal of endotoxins from antibodies and other proteins. These devices combine quality separations found in gravity columns with the speed of centrifugal separators. Using the unique FlowGo™ design, about 99% of endotoxins can be removed in a single pass through the agarose resin with protein recoveries of 85% or greater. High Capacity (HC) columns are available for recombinant proteins expressed from bacteria such as E. coli and Standard (S) columns are offered for purification of monoclonal antibodies from mammalian cells. Mini and Micro kits are available with loose resin for samples 20 ml or less in volume.
| Micro | Mini | Standard | High Capacity | |
| Typical in situ binding capacity | 300-500 EU | 3,000 EU | 30,000 EU | 500,000-1,000,000 EU |
| Typical binding capacity per ml resin | 500-800 EU/ml | 300 EU/ml | 1,500 EU/ml | 30,000 EU/ml |
| Minimum endotoxin levels tested post-column | <0.03 EU/ml | <0.03 EU/ml | <0.05 EU/ml | <0.05 EU/ml |
| Typical endotoxin clearance after 1 pass | 3 log reduction | 3 log reduction | ||
| Typical endotoxin clearance after 2 passes | 4 log reduction | 4 log reduction | ||
| Typical endotoxin clearance after 1 hour incubation | 3 log reduction | 2 log reduction | ||
| Typical endotoxin clearance after 3 hour incubation | 4 log reduction | 3 log reduction | ||
| Maximum sample load volume | 0.6 ml | 20 ml | 20 ml | 20 ml |
| Bed volume | 0.01-0.1 ml loose | 0.25 ml loose | 1 ml pre-packed | 1.7 ml pre-packed |
| Bead size range | 100 µm | 100 µm | 100 µm | 100 µm |
| Recommended working pH | 4-8 | 4-8 | 4-8 | 4-8 |
| Color coded end-caps | Supplied in vials | Supplied in vials | Light green | Dark green |
| NoEndo™ S, HC & M | NoEndo™ Micro | |
| Sample Capacity (by rotor type) | ||
| Swinging bucket | 20 ml | NOT USABLE |
| Fixed angle | 12 ml (25°) | 0.6 ml |
| Dimensions | ||
| Length | 118 mm | 48 mm |
| Width | 29 mm | 11 mm |
| Hold-up volume | 10 µl | 2-3 µl |
| Materials of Construction | ||
| Outer tube | Polypropylene | Polypropylene |
| Inner tube | Polyethylene | Polypropylene |
| Resin sintered plug | Polyethylene | N/A |
| NoEndo™ resin | Cross-linked 6% agarose | Cross-linked 6% agarose |
| Centrifuge Required | ||
| Rotor cavity | To fit 50 ml conical bottom tubes | To fit 2.2 ml centrifuge tubes |
| Maximum Speed |
1,500 x g (Fixed angle or Swinging Bucket) |
13,000 x g (Fixed angle ONLY) |
| Description | Prod. # | Price | Buy |
|---|---|---|---|
|
2 spin columns, 4 centrifuge tubes, 1 vial of resin (0.2 ml)
SKU: GEN-NoE2MICRO
Price: $83.00 |
Add to Cart | ||
|
2 spin columns, 2 centrifuge tubes, 2 vials of resin (0.25 ml each), 2 incubation caps
SKU: GEN-NoE2M
Price: $125.00 |
Add to Cart | ||
|
2 spin columns, 2 centrifuge tubes, 2 standard resin cartridges, 1 insertion tool
SKU: GEN-NoE2S
Price: $142.00 |
Add to Cart | ||
|
2 spin columns, 2 centrifuge tubes, 2 high capacity resin cartridges, 1 insertion tool
SKU: GEN-NoE2HC
Price: $160.00 |
Add to Cart | ||
|
12 spin columns, 12 centrifuge tubes, 12 standard resin cartridges, 1 insertion tool
SKU: GEN-NoE12S
Price: $555.00 |
Add to Cart | ||
|
12 spin columns, 12 centrifuge tubes, 12 high capacity resin cartridges, 1 insertion tool
SKU: GEN-NoE12HC
Price: $655.00 |
Add to Cart | ||
|
12 spin columns, 12 centrifuge tubes, 12 vials of resin (0.25 ml each), 12 incubation caps
SKU: GEN-NoE12M
Price: $435.00 |
Add to Cart | ||
|
24 spin columns, 48 centrifuge tubes, 1 vial of resin (2.4 ml)
SKU: GEN-NoE24MICRO
Price: $360.00 |
Add to Cart | ||
|
48 spin columns, 48 standard resin cartridges, 1 insertion tool (no centrifuge tubes)
SKU: GEN-NoE48S
Price: $1,750.00 |
Add to Cart | ||
|
48 spin columns, 48 high capacity resin cartridges, 1 insertion tool (no centrifuge tubes)
SKU: GEN-NoE48HC
Price: $2,000.00 |
Add to Cart | ||
|
100ml NoEndo loose resin suspended in 20% ethanol
SKU: GEN-NoE100ML
Price: $1,760.00 |
Add to Cart |
