Vivaspin® Filtrate Concentrator

Vivaspin® Filtrate Concentrator

Up to 2.5 ml starting volume
Vivaspin® Filtrate ultrafiltration concentrators are disposable devices for biological samples. Vivaspin® Filtrate is suitable for sample volumes up to 2.5 ml. The unique design allows ultrafiltration to take place in the opposite direction to the centrifugal force. This minimizes membrane blockage even with whole blood or cell lysate samples. The filtrate is collected in the floater insert tube where it is easily withdrawn.

Typical applications include:

  • Concentration of fungal antibodies prior to complement fixation or immunodiffusion for detection of Coccidioides and Aspergillus.
  • Concentrate bacterial antigens (H. influenzae, S. pneumoniae) prior to latex particle agglutination for detection of sepsis in newborns.
  • Protein removal from serum & cell / tissue lysates.
  • Prepare samples for mass spectrometry.

Performance Characteristics

Time to filter
50% of sample
Time to filter
90% of sample
Passage of
sample species
BSA 1.0 mg / ml (66,000 MW)
5,000 MWCO CTA 300 min N / A 0%
10,000 MWCO CTA 35 min 80 min 2%
20,000 MWCO CTA 9 min 20 min 2%
IgG 0.25 mg / ml (160,000 MW)
100,000 MWCO PES 13 min 35 min 3%
Blue Dextran 0.1 mg / ml (2,000,000 MW)
300,000 MWCO PES 9 min 25 min 28%

* 2.5 ml samples were loaded into each device.
The devices were centrifuged at 2,000 g until the required filtrate volumes had been reached.

Tech Specs

Concentrator Capacity
Swing Bucket Rotor 2.5 ml
Fixed Angle Rotor 2.5 ml
Total Length 93 mm
Width 14 mm
Active Membrane Area 0.79 cm²
Hold-up volume of membrane < 5 µl
Dead stop volume 100 µl
Materials of Construction
Centrifuge tube Polystyrene
Floater tube Styrene acrylonitrile
Cap Polyethylene
Membrane CTA, PES

Equipment Required

Rotor Type Swing bucket Fixed Angle
Minimum rotor angle - 25°
Rotor Cavity To fit 15 ml (17 mm) conical bottom tubes To fit 15 ml (17 mm) conical bottom tubes
Maximum Speed 2,500 g 2,000 g
Concentrate Recovery
Pipette type Fixed or variable volume Fixed or variable volume
Recommended tip Thin gel loader type Thin gel loader type

Ordering Information

Description Prod. No. Price
5,000 MWCO, Cellulose Triacetate Membrane, 12 per pack 13229-E $97.20
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10,000 MWCO, Cellulose Triacetate Membrane, 12 per pack 13239-E $97.20
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20,000 MWCO, Cellulose Triacetate Membrane, 12 per pack 13249-E $97.20
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100,000 MWCO, Polyethersulfone Membrane, 12 per pack 13269-E $97.20
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300,000 MWCO, Polyethersulfone Membrane, 12 per pack 13279-E $97.20
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