Vivaspin® Filtrate Concentrator
Up to 2.5 ml starting volume
Vivaspin® Filtrate ultrafiltration concentrators are disposable devices for biological samples. Vivaspin® Filtrate is suitable for sample volumes up to 2.5 ml. The unique design allows ultrafiltration to take place in the opposite direction to the centrifugal force. This minimizes membrane blockage even with whole blood or cell lysate samples. The filtrate is collected in the floater insert tube where it is easily withdrawn.
Typical applications include:
- Concentration of fungal antibodies prior to complement fixation or immunodiffusion for detection of Coccidioides and Aspergillus.
- Concentrate bacterial antigens (H. influenzae, S. pneumoniae) prior to latex particle agglutination for detection of sepsis in newborns.
- Protein removal from serum & cell / tissue lysates.
- Prepare samples for mass spectrometry.
Performance Characteristics
|
Time to filter 50% of sample volume |
Time to filter 90% of sample volume |
Passage of sample species volume |
|
| BSA 1.0 mg / ml (66,000 MW) | |||
| 5,000 MWCO CTA | 300 min | N / A | 0% |
| 10,000 MWCO CTA | 35 min | 80 min | 2% |
| 20,000 MWCO CTA | 9 min | 20 min | 2% |
| IgG 0.25 mg / ml (160,000 MW) | |||
| 100,000 MWCO PES | 13 min | 35 min | 3% |
| Blue Dextran 0.1 mg / ml (2,000,000 MW) | |||
| 300,000 MWCO PES | 9 min | 25 min | 28% |
* 2.5 ml samples were loaded into each device.
The devices were centrifuged at 2,000 g until the required filtrate volumes had been reached.
Tech Specs
| Concentrator Capacity | |
| Swing Bucket Rotor | 2.5 ml |
| Fixed Angle Rotor | 2.5 ml |
| Dimensions | |
| Total Length | 93 mm |
| Width | 14 mm |
| Active Membrane Area | 0.79 cm² |
| Hold-up volume of membrane | < 5 µl |
| Dead stop volume | 100 µl |
| Materials of Construction | |
| Centrifuge tube | Polystyrene |
| Floater tube | Styrene acrylonitrile |
| Cap | Polyethylene |
| Membrane | CTA, PES |
Equipment Required
| Centrifuge | ||
| Rotor Type | Swing bucket | Fixed Angle |
| Minimum rotor angle | - | 25° |
| Rotor Cavity | To fit 15 ml (17 mm) conical bottom tubes | To fit 15 ml (17 mm) conical bottom tubes |
| Maximum Speed | 2,500 g | 2,000 g |
| Concentrate Recovery | ||
| Pipette type | Fixed or variable volume | Fixed or variable volume |
| Recommended tip | Thin gel loader type | Thin gel loader type |
Ordering Information
| Description | Prod. No. | Price | |
| 5,000 MWCO, Cellulose Triacetate Membrane, 12 per pack | 13229-E |
$97.20 |
Add to Cart |
| 10,000 MWCO, Cellulose Triacetate Membrane, 12 per pack | 13239-E |
$97.20 |
Add to Cart |
| 20,000 MWCO, Cellulose Triacetate Membrane, 12 per pack | 13249-E |
$97.20 |
Add to Cart |
| 100,000 MWCO, Polyethersulfone Membrane, 12 per pack | 13269-E |
$97.20 |
Add to Cart |
| 300,000 MWCO, Polyethersulfone Membrane, 12 per pack | 13279-E |
$97.20 |
Add to Cart |
How to Order
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