Vivaspin 15 Centrifugal Concentrator
2 - 15 ml samples
The Vivaspin 15 concentrator is a disposable ultrafiltration device for use in swing bucket centrifuges accommodating 50 ml tubes. Vivaspin 15 is used for the concentration
of biological samples in the 2-15 ml range. The innovative design (US Patent no. 5,647,990, second patent pending), simplicity, speed and exceptional concentrate recoveries are the main
features of the concentrator.
In a single spin, 15 ml solutions can be concentrated up to 300x. Samples can be typically concentrated in 10-30 minutes with macromolecular recoveries in excess of 95%. The longitudinal membrane location and adjacent thin channel, provide optimum cross flow conditions even for particle laden solutions, the centrifugal force pulling particles and solids away from the membrane to the bottom of the device. Macromolecules collect in an impermeable 50 µl concentrate pocket integrally moulded below the membrane surface, thereby eliminating the risk of filtration to dryness.
|
Time to concentrate up to 30x [min.] at 20°C |
Concentrate Recovery % |
|
| Rotor | Fixed angle | |
| Centrifugal force | 2,000 x g | |
| Start volume | 15 ml | |
| Min. | Rec. | |
| BSA 1.0 mg / ml (66,000 MW) | ||
| 5,000 MWCO | 40 | 97% |
| 10,000 MWCO | 25 | 97% |
| 30,000 MWCO | 25 | 96% |
| 50,000 MWCO | 25 | 96% |
| 100,000 MWCO | 15 | 70% |
| Cytochrome c 0.25 mg / ml (12,400 MW) | ||
| 5,000 MWCO | 55 | 97% |
| 10,000 MWCO | 45 | 95% |
| 30,000 MWCO | 45 | 59% |
| 50,000 MWCO | 45 | 40% |
| 100,000 MWCO | 20 | 16% |
| IgG 0.25 mg / ml (160,000 MW) | ||
| 30,000 MWCO PES | 30 | 94% |
| 50,000 MWCO PES | 30 | 94% |
| 100,000 MWCO PES | 30 | 90% |
| Yeast 1.0 mg / ml (S. Cerevisiae) | ||
| 100,000 MWCO | 15 | 98% |
| 0.2 µm PES | 07 | 95% |
| Concentrator Capacity | |
| Swing bucket rotor | 15 ml |
| Fixed angle rotor | 8 ml |
| Dimensions | |
| Total Length | 76 mm |
| Width | 25.5 mm |
| Active Membrane Area | 4 cm² |
| Hold-up volume, membrane and support | <20 µl |
| Dead stop volume | 50 µl |
| Materials of Construction | |
| Body | Polycarbonate |
| Filtrate Vessel | Polypropylene |
| Concentrator Cap | Polycarbonate |
| Membrane | Polyethersulfone |
| Centrifuge | ||
| Rotor Type | Swing bucket | Fixed angle |
| Minimum rotor angle | - | 25° |
| Rotor Cavity | To fit 50 ml (17 mm) conical bottom tubes | To fit 50 ml (17 mm) conical bottom tubes |
| Maximum Speed | 3,000 g* | 3,000 g |
| Concentrate Recovery | ||
| Pipette type | Fixed or variable volume | Fixed or variable volume |
| Recommended tip | Thin gel loader type | Thin gel loader type |
* Please note, devices with membrane MWCO > 100 kDa need to be processed at lower g forces. See data sheet for details.
| Vivaspin 15 Polyethersulfone | Pack Size | Prod. No. | |
| 5,000 MWCO | 10 | VS1511 | BUY |
| 40 | VS1512 | BUY | |
| 10,000 MWCO | 10 | VS1501 | BUY |
| 40 | VS1502 | BUY | |
| 30,000 MWCO | 10 | VS1521 | BUY |
| 40 | VS1522 | BUY | |
| 50,000 MWCO | 10 | VS1531 | BUY |
| 40 | VS1532 | BUY | |
| 100,000 MWCO | 10 | VS1541 | BUY |
| 40 | VS1542 | BUY | |
| 0.2 µm | 10 | VS1571 | BUY |
| 40 | VS1572 | BUY | |
| Starter pack (5 of each 5k, 10k, 30k, 50k, 100k) | 10 | VS15S1 | BUY |
| Accessories | |||
| Conical bottom 50 ml tubes and lids | 100 | VSA001 | BUY |