Vivaspin® 15 Centrifugal Concentrator
2 - 15 ml samples
The Vivaspin® 15 concentrator is a disposable ultrafiltration device for use in swing bucket centrifuges accommodating 50 ml tubes. Vivaspin® 15 is used for the concentration
of biological samples in the 2-15 ml range. The innovative design (US Patent no. 5,647,990, second patent pending), simplicity, speed and exceptional concentrate recoveries are the main
features of the concentrator.
In a single spin, 15 ml solutions can be concentrated up to 300x. Samples can be typically concentrated in 10-30 minutes with macromolecular recoveries in excess of 95%. The longitudinal membrane location and adjacent thin channel, provide optimum cross flow conditions even for particle laden solutions, the centrifugal force pulling particles and solids away from the membrane to the bottom of the device. Macromolecules collect in an impermeable 50 µl concentrate pocket integrally moulded below the membrane surface, thereby eliminating the risk of filtration to dryness.
Applications
- Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis.
- Concentrate spinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis.
- Free drug & hormone (T3 / T4) analysis. Process samples in about 30 minutes instead of 17 hrs for equilibrium dialysis.
- Protein removal from serum & cell / tissue lysates.
- Exchange buffers for proteomic peptide samples.
- Prepare samples for mass spectrometry.
|
Time to concentrate up to 30x [min.] at 20°C |
Concentrate Recovery % |
|
| Rotor | Swing Bucket | |
| Centrifugal force | 2,000 x g | |
| Start volume | 15 ml | |
| Min. | Rec. | |
| BSA 1.0 mg / ml (66,000 MW) | ||
| 5,000 MWCO PES | 40 | 97% |
| 10,000 MWCO PES | 25 | 97% |
| 30,000 MWCO PES | 25 | 96% |
| 50,000 MWCO PES | 25 | 96% |
| 100,000 MWCO PES | 15 | 70% |
| Cytochrome c 0.25 mg / ml (12,400 MW) | ||
| 5,000 MWCO PES | 55 | 97% |
| 10,000 MWCO PES | 45 | 95% |
| 30,000 MWCO PES | 45 | 59% |
| 50,000 MWCO PES | 45 | 40% |
| 100,000 MWCO PES | 20 | 16% |
| IgG 0.25 mg / ml (160,000 MW) | ||
| 30,000 MWCO PES | 30 | 94% |
| 50,000 MWCO PES | 30 | 94% |
| 100,000 MWCO PES | 30 | 90% |
| Yeast 1.0 mg / ml (S. Cerevisiae) | ||
| 100,000 MWCO PES | 15 | 98% |
| 0.2 µm PES | 07 | 95% |
| Concentrator Capacity | |
| Swing bucket rotor | 15 ml |
| Fixed angle rotor | 8 ml |
| Dimensions | |
| Total Length | 76 mm |
| Width | 25.5 mm |
| Active Membrane Area | 4 cm² |
| Hold-up volume, membrane and support | <20 µl |
| Dead stop volume | 50 µl |
| Materials of Construction | |
| Body | Polycarbonate |
| Filtrate Vessel | Polypropylene |
| Concentrator Cap | Polycarbonate |
| Membrane | Polyethersulfone |
| Centrifuge | ||
| Rotor Type | Swing bucket | Fixed angle |
| Minimum rotor angle | - | 25° |
| Rotor Cavity | To fit 50 ml (30 mm) conical bottom tubes** | To fit 50 ml (30 mm) conical bottom tubes** |
| Maximum Speed | 3,000 g* | 3,000 g |
| Concentrate Recovery | ||
| Pipette type | Fixed or variable volume | Fixed or variable volume |
| Recommended tip | Thin gel loader type | Thin gel loader type |
* Please note, devices with membrane MWCO ≥ 100 kDa need to be processed at lower g forces. See data sheet for details.
** Vivaspin® 15 can also fit 29 x 104 mm round bottom tubes.
| Description | Prod. No. | Price | |
| 5,000 MWCO, PES Membrane, 10 per pack | VS1511 |
$77.00 |
Add to Cart |
| 5,000 MWCO, PES Membrane, 40 per pack | VS1512 |
$277.00 |
Add to Cart |
| 10,000 MWCO, PES Membrane, 10 per pack | VS1501 |
$77.00 |
Add to Cart |
| 10,000 MWCO, PES Membrane, 40 per pack | VS1502 |
$277.00 |
Add to Cart |
| 30,000 MWCO, PES Membrane, 10 per pack | VS1521 |
$77.00 |
Add to Cart |
| 30,000 MWCO, PES Membrane, 40 per pack | VS1522 |
$277.00 |
Add to Cart |
| 50,000 MWCO, PES Membrane, 10 per pack | VS1531 |
$77.00 |
Add to Cart |
| 50,000 MWCO, PES Membrane, 40 per pack | VS1532 |
$277.00 |
Add to Cart |
| 100,000 MWCO, PES Membrane, 10 per pack | VS1541 |
$77.00 |
Add to Cart |
| 100,000 MWCO, PES Membrane, 40 per pack | VS1542 |
$277.00 |
Add to Cart |
| PES Membrane, Start Pack with 5@ 5K/10K/30K/50K/100K MWCO | VS15S1 |
$70.00 |
Add to Cart |