Vivaspin® 2 Centrifugal Concentrator

Vivaspin® 2 Centrifugal Concentrator

Vivaspin® 0.4 to 2 ml
The new Vivaspin® 2 bridges the gap between the 500 µl and 4 ml centrifugal concentrators. This device combines the speed of the classic Vivaspin® products with low internal surface and membrane area for superior recoveries from very dilute solutions.

Available with a choice of polyethersulfone, cellulose triacetate and Hydrosart® membranes, Vivaspin® 2 offers the highest flexibility for process optimisation.

Also unique to the Vivaspin® 2, is the choice of directly pipetting the concentrate from the dead stop pocket built into the bottom of the concentrator, or alternatively reverse spinning into the concentrate recovery cap which can then be sealed for storage.

Both methods result in near total concentrate recoveries.

Applications

  • Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis.
  • Concentrate spinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis.
  • Free drug & hormone (T3 / T4) analysis. Process samples in about 30 minutes instead of 17 hrs for equilibrium dialysis.
  • Protein removal from serum & cell / tissue lysates.
  • Exchange buffers for proteomic peptide samples.
  • Prepare samples for mass spectrometry.
Performance Characteristics
Time to concentrate
up to 30x [min.] at 20°C
Concentrate
Recovery %
Centrifugal force 5,000 x g 5,000 x g
Insulin chain A 0.1mg/ml (2,535 MW)
2,000 MWCO HY 35 95%
Aprotinin 0.25mg/ml (6,500 MW)
3,000 MWCO PES 50 96%
BSA 1.0 mg / ml (66,000 MW)
5,000 MWCO PES 12 98%
5,000 MWCO CTA 50 96%
10,000 MWCO PES 8 98%
10,000 MWCO CTA 10 96%
10,000 MWCO HY 12 98%
20,000 MWCO CTA 5 96%
30,000 MWCO PES 8 97%
30,000 MWCO HY 5 97%
IgG 0.25 mg / ml (160,000 MW)
20,000 MWCO CTA 6 97%
30,000 MWCO PES 10 96%
50,000 MWCO PES 10 95%
100,000 MWCO PES 8 95%
Tech Specs
Concentrator Capacity
Swing Bucket Rotor 3 ml
Fixed Angle Rotor 2 ml
Dimensions
Total Length 126 mm
Width 17 mm
Active Membrane Area 1.2 cm²
Hold-up volume of membrane <10 µl
Dead stop volume 8 µl
Materials of Construction
Body Polycarbonate
Filtrate Vesel Polycarbonate
Concentrator Cap Polycarbonate
Membrane Polyethersulfone
Cellulose Triacetate
Hydrosart®
Equipment Required
Centrifuge
Rotor Type Swing bucket Fixed Angle
Minimum rotor angle - 25°
Rotor Cavity To fit 15 ml (17 mm) conical bottom tubes To fit 15 ml (17 mm) conical bottom tubes
Maximum Speed 4,000 g 12,000 g*
Concentrate Recovery
Pipette type Fixed or variable volume Fixed or variable volume
Recommended tip Thin gel loader Thin gel loader

* Please note, devices with membrane types CTA, HY and PES MWCO ≥ 100 kDa need to be processed at lower g forces. See data sheet for details.

Ordering Information
Description Prod. No. Price
PES Membrane, 3,000 MWCO, 25 per Pack VS0291 $102.00
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PES Membrane, 3,000 MWCO, 100 per Pack VS0292 $355.00
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PES Membrane, 5,000 MWCO, 25 per Pack VS0211 $102.00
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PES Membrane, 5,000 MWCO, 100 per Pack VS0212 $355.00
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PES Membrane, 10,000 MWCO, 25 per Pack VS0201 $102.00
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PES Membrane, 10,000 MWCO, 100 per Pack VS0202 $355.00
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PES Membrane, 30,000 MWCO, 25 per Pack VS0221 $102.00
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PES Membrane, 30,000 MWCO, 100 per Pack VS0222 $355.00
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PES Membrane, 50,000 MWCO, 25 per Pack VS0231 $102.00
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PES Membrane, 50,000 MWCO, 100 per Pack VS0232 $355.00
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PES Membrane, 100,000 MWCO, 25 per Pack VS0241 $102.00
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PES Membrane, 100,000 MWCO, 100 per Pack VS0242 $355.00
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PES Membrane, 300,000 MWCO, 25 per Pack VS0251 $102.00
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PES Membrane, 300,000 MWCO, 100 per Pack VS0252 $355.00
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PES Membrane, 1,000,000 MWCO, 25 per Pack VS0261 $102.00
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PES Membrane, 1,000,000 MWCO, 100 per Pack VS0262 $355.00
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PES Membrane, 0.2µm MWCO, 25 per Pack VS0271 $102.00
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PES Membrane, 0.2µm MWCO, 100 per Pack VS0272 $355.00
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PES Membrane, Start Pack with 5@ 5K/10K/30K/50K/100K MWCO VS02S1 $102.00
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Hydrosart Membrane, 2,000 MWCO, 25 per Pack VS02H91 $110.00
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Hydrosart Membrane, 2,000 MWCO, 100 per Pack VS02H92 $367.00
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Hydrosart Membrane, 5,000 MWCO, 25 per Pack VS02H11 $110.00
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Hydrosart Membrane, 5,000 MWCO, 100 per Pack VS02H12 $367.00
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Hydrosart Membrane, 10,000 MWCO, 25 per Pack VS02H01 $110.00
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Hydrosart Membrane, 10,000 MWCO, 100 per Pack VS02H02 $367.00
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Hydrosart Membrane, 30,000 MWCO, 25 per Pack VS02H21 $110.00
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Hydrosart Membrane, 30,000 MWCO, 100 per Pack VS02H22 $367.00
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CTA Membrane, 5,000 MWCO, 25 per Pack VS02U1 $102.00
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CTA Membrane, 5,000 MWCO, 100 per Pack VS02U2 $355.00
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CTA Membrane, 10,000 MWCO, 25 per Pack VS02V1 $102.00
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CTA Membrane, 10,000 MWCO, 100 per Pack VS02V2 $355.00
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CTA Membrane, 20,000 MWCO, 25 per Pack VS02X1 $102.00
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CTA Membrane, 20,000 MWCO, 100 per Pack VS02X2 $355.00
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