Vivaspin® 15 Turbo Centrifugal Concentrator

Vivaspin® 15 Turbo Centrifugal Concentrator

Concentrate up to 150x

The Vivaspin® Turbo 15 concentrator is a disposable ultrafiltration device for use in swinging bucket and fixed angle centrifuges accommodating 50 ml conical bottom tubes. Vivaspin® Turbo 15 is used for the concentration of biological sample volumes up to 15 ml. The innovative design (US Patent no. 5,647,990, second patent pending), simplicity, speed and exceptional concentrate recoveries are the main features of the concentrator.

In a single spin, 15 ml solutions can be concentrated up to 150x. Samples can be typically concentrated in 10-30 minutes with macromolecular recoveries in excess of 95%. The twin vertical membranes and adjacent thin channel, provide optimum cross flow conditions even for particle laden solutions. The centrifugal force pulls particles and solids away from the membrane to the bottom of the device. Macromolecules collect in a 100 µl concentrate pocket which is uniquely angled and molded below the membrane surface, thereby eliminating the risk of filtration to dryness.

Applications

  • Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis.
  • Concentrate spinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis.
  • Protein removal from serum & cell / tissue lysates.
  • Exchange buffers for proteomic peptide samples.
  • Prepare samples for mass spectrometry.

Performance Characteristics

Time to concentrate up to 20x [min.] at 20°C and solute recovery %
Rotor Swing Bucket Fixed Angle
Centrifugal force 4,000 x g 4,000 x g
Start volume 15 ml 11 ml
Min. Rec. Min. Rec.
Cytochrome c 0.25 mg/ml (12,400 MW)
5,000 MWCO PES 30 98% 50 98%
Lysozyme 0.25 mg/ml (14,300 MW)
5,000 MWCO PES 33 96% 50 96%
α-Chymotrypsin 1 mg/ml (25,000 MW)
10,000 MWCO PES 10 95% 10 95%
BSA 1 mg/ml (66,000 MW)
10,000 MWCO PES 10 99% 10 99%
30,000 MWCO PES 8 98% 10 98%
IgG 1 mg/ml (160,000 MW)
30,000 MWCO PES 23 95% 17 95%

Tech Specs

Concentrator Capacity
Swinging bucket rotor 15 ml
Fixed angle rotor 11 ml
Dimensions
Total Length 118 mm
Width 27 mm
Active Membrane Area 7.2 cm²
Hold-up volume, membrane and support <10 µl
Dead stop volume (swinging bucket) 100 µl
Dead stop volume (fixed angle) 60 µl
Materials of Construction
Body Styrene butadiene copolymer
Filtrate Vessel Polypropylene
Concentrator Cap Polypropylene
Membrane Polyethersulfone

Equipment Required

Centrifuge
Rotor Type Swing bucket Fixed angle
Minimum rotor angle - 25°
Rotor Cavity To fit 50 ml (30 mm) conical bottom tubes** To fit 50 ml (30 mm) conical bottom tubes**
Maximum Speed 3,000 g 3,000 g
Concentrate Recovery
Pipette type Fixed or variable volume Fixed or variable volume
Standard 200 µl tip Thin gel loader type Thin gel loader type

** Vivaspin® 15 can also fit 29 x 104 mm round bottom tubes.

Vivaspin® 15 Turbo Ordering Information

Description Prod. No. Price
3,000 MWCO, PES Membrane, 12 per pack VS15T91 $149.00
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3,000 MWCO, PES Membrane, 48 per pack VS15T92 $565.00
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5,000 MWCO, PES Membrane, 12 per pack VS15T11 $149.00
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5,000 MWCO, PES Membrane, 48 per pack VS15T12 $565.00
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10,000 MWCO, PES Membrane, 12 per pack VS15T01 $149.00
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10,000 MWCO, PES Membrane, 48 per pack VS15T02 $565.00
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30,000 MWCO, PES Membrane, 12 per pack VS15T21 $149.00
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30,000 MWCO, PES Membrane, 48 per pack VS15T22 $565.00
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50,000 MWCO, PES Membrane, 12 per pack VS15T31 $149.00
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50,000 MWCO, PES Membrane, 48 per pack VS15T32 $565.00
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100,000 MWCO, PES Membrane, 12 per pack VS15T41 $149.00
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100,000 MWCO, PES Membrane, 48 per pack VS15T42 $565.00
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