Vivaspin® 15 Turbo Centrifugal Concentrator
Concentrate up to 150x
The Vivaspin® Turbo 15 concentrator is a disposable ultrafiltration device for use in swinging bucket and fixed angle centrifuges accommodating 50 ml conical bottom tubes. Vivaspin® Turbo 15 is used for the concentration of biological sample volumes up to 15 ml. The innovative design (US Patent no. 5,647,990, second patent pending), simplicity, speed and exceptional concentrate recoveries are the main features of the concentrator.
In a single spin, 15 ml solutions can be concentrated up to 150x. Samples can be typically concentrated in 10-30 minutes with macromolecular recoveries in excess of 95%. The twin vertical membranes and adjacent thin channel, provide optimum cross flow conditions even for particle laden solutions. The centrifugal force pulls particles and solids away from the membrane to the bottom of the device. Macromolecules collect in a 100 µl concentrate pocket which is uniquely angled and molded below the membrane surface, thereby eliminating the risk of filtration to dryness.
Applications
- Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis.
- Concentrate spinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis.
- Protein removal from serum & cell / tissue lysates.
- Exchange buffers for proteomic peptide samples.
- Prepare samples for mass spectrometry.
Performance Characteristics
| Rotor | Swing Bucket | Fixed Angle | ||
| Centrifugal force | 4,000 x g | 4,000 x g | ||
| Start volume | 15 ml | 11 ml | ||
| Min. | Rec. | Min. | Rec. | |
| Cytochrome c 0.25 mg/ml (12,400 MW) | ||||
| 5,000 MWCO PES | 30 | 98% | 50 | 98% |
| Lysozyme 0.25 mg/ml (14,300 MW) | ||||
| 5,000 MWCO PES | 33 | 96% | 50 | 96% |
| α-Chymotrypsin 1 mg/ml (25,000 MW) | ||||
| 10,000 MWCO PES | 10 | 95% | 10 | 95% |
| BSA 1 mg/ml (66,000 MW) | ||||
| 10,000 MWCO PES | 10 | 99% | 10 | 99% |
| 30,000 MWCO PES | 8 | 98% | 10 | 98% |
| IgG 1 mg/ml (160,000 MW) | ||||
| 30,000 MWCO PES | 23 | 95% | 17 | 95% |
Tech Specs
| Concentrator Capacity | |
| Swinging bucket rotor | 15 ml |
| Fixed angle rotor | 11 ml |
| Dimensions | |
| Total Length | 118 mm |
| Width | 27 mm |
| Active Membrane Area | 7.2 cm² |
| Hold-up volume, membrane and support | <10 µl |
| Dead stop volume (swinging bucket) | 100 µl |
| Dead stop volume (fixed angle) | 60 µl |
| Materials of Construction | |
| Body | Styrene butadiene copolymer |
| Filtrate Vessel | Polypropylene |
| Concentrator Cap | Polypropylene |
| Membrane | Polyethersulfone |
Equipment Required
| Centrifuge | ||
| Rotor Type | Swing bucket | Fixed angle |
| Minimum rotor angle | - | 25° |
| Rotor Cavity | To fit 50 ml (30 mm) conical bottom tubes** | To fit 50 ml (30 mm) conical bottom tubes** |
| Maximum Speed | 4,000 g* | 4,000 g* |
| Concentrate Recovery | ||
| Pipette type | Fixed or variable volume | Fixed or variable volume |
| Standard 200 µl tip | Thin gel loader type | Thin gel loader type |
* Please note, devices with membrane MWCO ≥ 100 kDa need to be processed at lower g forces. See data sheet for details.
** Vivaspin® 15 can also fit 29 x 104 mm round bottom tubes.
Vivaspin® 15 Turbo Ordering Information
| Description | Prod. No. | Price | |
| 3,000 MWCO, PES Membrane, 12 per pack | VS15T91 |
$185.00 |
Add to Cart |
| 3,000 MWCO, PES Membrane, 48 per pack | VS15T92 |
$706.00 |
Add to Cart |
| 5,000 MWCO, PES Membrane, 12 per pack | VS15T11 |
$185.00 |
Add to Cart |
| 5,000 MWCO, PES Membrane, 48 per pack | VS15T12 |
$706.00 |
Add to Cart |
| 10,000 MWCO, PES Membrane, 12 per pack | VS15T01 |
$185.00 |
Add to Cart |
| 10,000 MWCO, PES Membrane, 48 per pack | VS15T02 |
$706.00 |
Add to Cart |
| 30,000 MWCO, PES Membrane, 12 per pack | VS15T21 |
$185.00 |
Add to Cart |
| 30,000 MWCO, PES Membrane, 48 per pack | VS15T22 |
$706.00 |
Add to Cart |
| 50,000 MWCO, PES Membrane, 12 per pack | VS15T31 |
$185.00 |
Add to Cart |
| 50,000 MWCO, PES Membrane, 48 per pack | VS15T32 |
$706.00 |
Add to Cart |
| 100,000 MWCO, PES Membrane, 12 per pack | VS15T41 |
$185.00 |
Add to Cart |
| 100,000 MWCO, PES Membrane, 48 per pack | VS15T42 |
$706.00 |
Add to Cart |