Vivaspin® 2 Centrifugal Concentrator

Vivaspin® 2 Centrifugal Concentrator

Up to 2 ml starting volume
Concentrate up to 250x
The Vivaspin® 2 bridges the gap between the 500 µl and 4 ml centrifugal concentrators. This device combines the speed of the classic Vivaspin® products with low internal surface and membrane area for superior recoveries from very dilute solutions.

Available with a choice of polyethersulfone, cellulose triacetate and Hydrosart® membranes, Vivaspin® 2 offers the highest flexibility for process optimisation.

Also unique to the Vivaspin® 2, is the choice of directly pipetting the concentrate from the dead stop pocket built into the bottom of the concentrator, or alternatively reverse spinning into the concentrate recovery cap which can then be sealed for storage.

Both methods result in near total concentrate recoveries.

Applications

  • Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis.
  • Concentrate spinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis.
  • Protein removal from serum & cell / tissue lysates.
  • Exchange buffers for proteomic peptide samples.
  • Prepare samples for mass spectrometry.

Performance Characteristics

Time to concentrate
up to 30x [min.] at 20°C
Concentrate
Recovery %
Centrifugal force 5,000 x g 5,000 x g
Insulin chain A 0.1mg/ml (2,535 MW)
2,000 MWCO HY 35 95%
Aprotinin 0.25mg/ml (6,500 MW)
3,000 MWCO PES 50 96%
BSA 1.0 mg / ml (66,000 MW)
5,000 MWCO PES 12 98%
5,000 MWCO CTA 50 96%
10,000 MWCO PES 8 98%
10,000 MWCO CTA 10 96%
10,000 MWCO HY 12 98%
20,000 MWCO CTA 5 96%
30,000 MWCO PES 8 97%
30,000 MWCO HY 5 97%
IgG 0.25 mg / ml (160,000 MW)
20,000 MWCO CTA 6 97%
30,000 MWCO PES 10 96%
50,000 MWCO PES 10 95%
100,000 MWCO PES 8 95%

Tech Specs

Concentrator Capacity
Swing Bucket Rotor 3 ml
Fixed Angle Rotor 2 ml
Dimensions
Total Length 126 mm
Width 17 mm
Active Membrane Area 1.2 cm²
Hold-up volume of membrane <10 µl
Dead stop volume 8 µl
Materials of Construction
Body Polycarbonate
Filtrate Vesel Polycarbonate
Concentrator Cap Polycarbonate
Membrane Polyethersulfone
Cellulose Triacetate
Hydrosart®

Equipment Required

Centrifuge
Rotor Type Swing bucket Fixed Angle
Minimum rotor angle - 25°
Rotor Cavity To fit 15 ml (17 mm) conical bottom tubes To fit 15 ml (17 mm) conical bottom tubes
Maximum Speed 4,000 g 12,000 g*
Concentrate Recovery
Pipette type Fixed or variable volume Fixed or variable volume
Recommended tip Thin gel loader Thin gel loader

* Please note, devices with membrane types CTA, HY and PES MWCO ≥ 100 kDa need to be processed at lower g forces. See data sheet for details.

Ordering Information

Description Prod. No. Price
3,000 MWCO, PES Membrane, 25 per Pack VS0291 $131.00
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3,000 MWCO, PES Membrane, 100 per Pack VS0292 $460.00
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5,000 MWCO, PES Membrane, 25 per Pack VS0211 $131.00
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5,000 MWCO, PES Membrane, 100 per Pack VS0212 $460.00
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10,000 MWCO, PES Membrane, 25 per Pack VS0201 $131.00
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10,000 MWCO, PES Membrane, 100 per Pack
 
For larger quantities - please contact us for a quotation.
VS0202 $460.00
Add to Cart
30,000 MWCO, PES Membrane, 25 per Pack VS0221 $131.00
Add to Cart
30,000 MWCO, PES Membrane, 100 per Pack VS0222 $460.00
Add to Cart
50,000 MWCO, PES Membrane, 25 per Pack VS0231 $131.00
Add to Cart
50,000 MWCO, PES Membrane, 100 per Pack VS0232 $460.00
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100,000 MWCO, PES Membrane, 25 per Pack VS0241 $131.00
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100,000 MWCO, PES Membrane, 100 per Pack VS0242 $460.00
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300,000 MWCO, PES Membrane, 25 per Pack VS0251 $131.00
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300,000 MWCO, PES Membrane, 100 per Pack VS0252 $460.00
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1,000,000 MWCO, PES Membrane, 25 per Pack VS0261 $131.00
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1,000,000 MWCO, PES Membrane, 100 per Pack VS0262 $460.00
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0.2µm MWCO, PES Membrane, 25 per Pack VS0271 $131.00
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0.2µm MWCO, PES Membrane, 100 per Pack VS0272 $460.00
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PES Membrane, Start Pack with 5@ 5K/10K/30K/50K/100K MWCO VS02S1 $131.00
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2,000 MWCO, Hydrosart Membrane, 25 per Pack VS02H91 $131.00
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2,000 MWCO, Hydrosart Membrane, 100 per Pack VS02H92 $460.00
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5,000 MWCO, Hydrosart Membrane, 25 per Pack VS02H11 $131.00
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5,000 MWCO, Hydrosart Membrane, 100 per Pack VS02H12 $460.00
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10,000 MWCO, Hydrosart Membrane, 25 per Pack VS02H01 $131.00
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10,000 MWCO, Hydrosart Membrane, 100 per Pack VS02H02 $460.00
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30,000 MWCO, Hydrosart Membrane, 25 per Pack VS02H21 $131.00
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30,000 MWCO, Hydrosart Membrane, 100 per Pack VS02H22 $460.00
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5,000 MWCO, CTA Membrane, 25 per Pack VS02U1 $131.00
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5,000 MWCO, CTA Membrane, 100 per Pack VS02U2 $460.00
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10,000 MWCO, CTA Membrane, 25 per Pack VS02V1 $131.00
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10,000 MWCO, CTA Membrane, 100 per Pack VS02V2 $460.00
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20,000 MWCO, CTA Membrane, 25 per Pack VS02X1 $131.00
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20,000 MWCO, CTA Membrane, 100 per Pack VS02X2 $460.00
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