Vivaspin® 500 Centrifugal Concentrator
Up to 500 µl starting volume
Concentrate up to 100x
Vivaspin® 500 µl centrifugal filter units offer a simple, one step procedure for sample preparation.
They can effectively be used in a fixed angle rotors accepting 2.2 ml centrifuge tubes.
The patented vertical membrane design and thin channel filtration chamber (US 5,647,990), minimises membrane fouling and provides high speed concentrations, even with particle laden solutions.
Applications
- Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis.
- Concentrate spinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis.
- Protein removal from serum & cell / tissue lysates.
- Exchange buffers for proteomic peptide samples.
- Prepare samples for mass spectrometry.
Performance Characteristics
|
Time to concentrate up to 30x [min.] at 20°C |
Concentrate Recovery % | |
| Rotor | fixed angle | |
| Centrifugal force | 12,000 x g | |
| Start volume | 500 µl | |
| Aprotinin 0.25mg/ml (6,500 MW) | ||
| 3,000 MWCO PES | 30 | 96% |
| BSA 1.0 mg / ml (66,000 MW) | ||
| 5,000 MWCO PES | 15 | 96% |
| 10,000 MWCO PES | 5 | 96% |
| 30,000 MWCO PES | 5 | 95% |
| IgG 0.25 mg / ml (160,000 MW) | ||
| 30,000 MWCO PES | 10 | 96% |
| 50,000 MWCO PES | 10 | 96% |
| 100,000 MWCO PES | 10 | 96% |
Tech Specs
| Concentrator Capacity | |
| Swing Bucket Rotor | do not use |
| Fixed Angle Rotor | 500 µl |
| Dimensions | |
| Total Length | 50 mm |
| Width | 11 mm |
| Active Membrane Area | 0.5 cm² |
| Hold-up volume, membrane and support | <5 µl |
| Dead stop volume | 5 µl |
| Materials of Construction | |
| Body | Polycarbonate |
| Filtrate Vessel | Polypropylene |
| Concentrator Cap | Polycarbonate |
| Membrane | Polyethersulfone |
Equipment Required
| Centrifuge | |
| Rotor Type | Fixed angle |
| Minimum rotor angle | 40° |
| Rotor Cavity | To fit 2.2 ml (11 mm) conical bottom tubes |
| Maximum Speed | 12,000 g |
| Concentrate Recovery | |
| Pipette type | Fixed or variable volume |
| Recommended tip | Thin gel loader type |
Ordering Information
| Description | Prod. No. | Price | |
| 3,000 MWCO, PES Membrane, 25 per pack | VS0191 |
$146.00 |
Add to Cart |
| 3,000 MWCO, PES Membrane, 100 per pack | VS0192 |
$515.00 |
Add to Cart |
| 5,000 MWCO, PES Membrane, 25 per pack | VS0111 |
$146.00 |
Add to Cart |
| 5,000 MWCO, PES Membrane, 100 per pack | VS0112 |
$515.00 |
Add to Cart |
| 10,000 MWCO, PES Membrane, 25 per pack | VS0101 |
$146.00 |
Add to Cart |
|
10,000 MWCO, PES Membrane, 100 per pack For larger quantities - please contact us for a quotation. |
VS0102 |
$515.00 |
Add to Cart |
| 30,000 MWCO, PES Membrane, 25 per pack | VS0121 |
$146.00 |
Add to Cart |
| 30,000 MWCO, PES Membrane, 100 per pack | VS0122 |
$515.00 |
Add to Cart |
| 50,000 MWCO, PES Membrane, 25 per pack | VS0131 |
$146.00 |
Add to Cart |
| 50,000 MWCO, PES Membrane, 100 per pack | VS0132 |
$515.00 |
Add to Cart |
| 100,000 MWCO, PES Membrane, 25 per pack | VS0141 |
$146.00 |
Add to Cart |
| 100,000 MWCO, PES Membrane, 100 per pack | VS0142 |
$515.00 |
Add to Cart |
| 300,000 MWCO, PES Membrane, 25 per pack | VS0151 |
$146.00 |
Add to Cart |
| 300,000 MWCO, PES Membrane, 100 per pack | VS0152 |
$515.00 |
Add to Cart |
| 1,000,000 MWCO, PES Membrane, 25 per pack | VS0161 |
$146.00 |
Add to Cart |
| 1,000,000 MWCO, PES Membrane, 100 per pack | VS0162 |
$515.00 |
Add to Cart |
| 0.2µm, PES Membrane, 25 per pack | VS0171 |
$146.00 |
Add to Cart |
| 0.2µm, PES Membrane, 100 per pack | VS0172 |
$515.00 |
Add to Cart |
How to Order
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