Vivaspin® 500 Centrifugal Concentrator

Vivaspin® 500 Centrifugal Concentrator

Up to 500 µl starting volume
Concentrate up to 100x
Vivaspin® 500 µl centrifugal filter units offer a simple, one step procedure for sample preparation. They can effectively be used in a fixed angle rotors accepting 2.2 ml centrifuge tubes.

The patented vertical membrane design and thin channel filtration chamber (US 5,647,990), minimises membrane fouling and provides high speed concentrations, even with particle laden solutions.

Applications

  • Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis.
  • Concentrate spinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis.
  • Protein removal from serum & cell / tissue lysates.
  • Exchange buffers for proteomic peptide samples.
  • Prepare samples for mass spectrometry.

Performance Characteristics

Time to concentrate
up to 30x [min.] at 20°C
Concentrate Recovery %
Rotor fixed angle
Centrifugal force 12,000 x g
Start volume 500 µl
Aprotinin 0.25mg/ml (6,500 MW)
3,000 MWCO PES 30 96%
BSA 1.0 mg / ml (66,000 MW)
5,000 MWCO PES 15 96%
10,000 MWCO PES 5 96%
30,000 MWCO PES 5 95%
IgG 0.25 mg / ml (160,000 MW)
30,000 MWCO PES 10 96%
50,000 MWCO PES 10 96%
100,000 MWCO PES 10 96%

Tech Specs

Concentrator Capacity
Swing Bucket Rotor do not use
Fixed Angle Rotor 500 µl
Dimensions
Total Length 50 mm
Width 11 mm
Active Membrane Area 0.5 cm²
Hold-up volume, membrane and support <5 µl
Dead stop volume 5 µl
Materials of Construction
Body Polycarbonate
Filtrate Vessel Polypropylene
Concentrator Cap Polycarbonate
Membrane Polyethersulfone

Equipment Required

Centrifuge
Rotor Type Fixed angle
Minimum rotor angle 40°
Rotor Cavity To fit 2.2 ml (11 mm) conical bottom tubes
Maximum Speed 15,000 g
Concentrate Recovery
Pipette type Fixed or variable volume
Recommended tip Thin gel loader type

Ordering Information

Description Prod. No. Price
3,000 MWCO, PES Membrane, 25 per pack VS0191 $118.00
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3,000 MWCO, PES Membrane, 100 per pack VS0192 $415.00
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5,000 MWCO, PES Membrane, 25 per pack VS0111 $118.00
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5,000 MWCO, PES Membrane, 100 per pack VS0112 $415.00
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10,000 MWCO, PES Membrane, 25 per pack VS0101 $118.00
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10,000 MWCO, PES Membrane, 100 per pack
 
For larger quantities - please contact us for a quotation.
VS0102 $415.00
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30,000 MWCO, PES Membrane, 25 per pack VS0121 $118.00
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30,000 MWCO, PES Membrane, 100 per pack VS0122 $415.00
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50,000 MWCO, PES Membrane, 25 per pack VS0131 $118.00
Add to Cart
50,000 MWCO, PES Membrane, 100 per pack VS0132 $415.00
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100,000 MWCO, PES Membrane, 25 per pack VS0141 $118.00
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100,000 MWCO, PES Membrane, 100 per pack VS0142 $415.00
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300,000 MWCO, PES Membrane, 25 per pack VS0151 $118.00
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300,000 MWCO, PES Membrane, 100 per pack VS0152 $415.00
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1,000,000 MWCO, PES Membrane, 25 per pack VS0161 $118.00
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1,000,000 MWCO, PES Membrane, 100 per pack VS0162 $415.00
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0.2µm, PES Membrane, 25 per pack VS0171 $118.00
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0.2µm, PES Membrane, 100 per pack VS0172 $415.00
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PES Membrane, Start Pack with 5@ 5K/10K/30K/50K/100K MWCO VS01S1 $118.00
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