Centrisart® I Concentrator

Centrisart® I Concentrator

Up to 2.5 ml starting volume
Centrisart® I ultrafiltration concentrators are disposable devices for biological samples. Centrisart® I is suitable for sample volumes up to 2.5 ml. The unique design allows ultrafiltration to take place in the opposite direction to the centrifugal force. This minimizes membrane blockage even with whole blood or cell lysate samples. The filtrate is collected in the floater insert tube where it is easily withdrawn.

Typical applications include:

  • Concentration of fungal antibodies prior to complement fixation or immunodiffusion for detection of Coccidioides and Aspergillus.
  • Concentrate bacterial antigens (H. influenzae, S. pneumoniae) prior to latex particle agglutination for detection of sepsis in newborns.
  • Protein removal from serum & cell / tissue lysates.
  • Prepare samples for mass spectrometry.

Performance Characteristics

Time to filter
50% of sample
volume
Time to filter
90% of sample
volume
Passage of
sample species
volume
BSA 1.0 mg / ml (66,000 MW)
5,000 MWCO CTA 300 min N / A 0%
10,000 MWCO CTA 35 min 80 min 2%
20,000 MWCO CTA 9 min 20 min 2%
IgG 0.25 mg / ml (160,000 MW)
100,000 MWCO PES 13 min 35 min 3%
Blue Dextran 0.1 mg / ml (2,000,000 MW)
300,000 MWCO PES 9 min 25 min 28%

* 2.5 ml samples were loaded into each device.
The devices were centrifuged at 2,000 g until the required filtrate volumes had been reached.

Tech Specs

Concentrator Capacity
Swing Bucket Rotor 2.5 ml
Fixed Angle Rotor 2.5 ml
Dimensions
Total Length 93 mm
Width 14 mm
Active Membrane Area 0.79 cm²
Hold-up volume of membrane < 5 µl
Dead stop volume 100 µl
Materials of Construction
Centrifuge tube Polystyrene
Floater tube Styrene acrylonitrile
Cap Polyethylene
Membrane CTA, PES

Equipment Required

Centrifuge
Rotor Type Swing bucket Fixed Angle
Minimum rotor angle - 25°
Rotor Cavity To fit 15 ml (17 mm) conical bottom tubes To fit 15 ml (17 mm) conical bottom tubes
Maximum Speed 2,500 g 2,000 g
Concentrate Recovery
Pipette type Fixed or variable volume Fixed or variable volume
Recommended tip Thin gel loader type Thin gel loader type

Ordering Information

Description Prod. No. Price
5,000 MWCO, Cellulose Triacetate Membrane, 12 per pack 13229-E $81.00
Add to Cart
10,000 MWCO, Cellulose Triacetate Membrane, 12 per pack 13239-E $81.00
Add to Cart
20,000 MWCO, Cellulose Triacetate Membrane, 12 per pack 13249-E $81.00
Add to Cart
100,000 MWCO, Polyethersulfone Membrane, 12 per pack 13269-E $81.00
Add to Cart
300,000 MWCO, Polyethersulfone Membrane, 12 per pack 13279-E $81.00
Add to Cart
3 each of 5K/10K/20K MWCO (CTA Membrane), 100K MWCO (PES Membrane), 13209-E $81.00
Add to Cart

How to Order

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* Start pack comes with 3 units each of 5k CTA, 10k CTA, 20k CTA and 100k PES)

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