Vivacon® 2 ETO
PCR Grade Vivacons achieved by Ethylene Oxide Treatment
The new Vivacon® 2 PCR Grade units, in addition to all features described below for Vivacon® 2, have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 10-6 and free of detectable human genomic DNA contamination tested with a qPCR assay over 55 cycles with a verified limit of detection of < 2 pg.
Vivacon® 2 are centrifugal concentrators for DNA concentration and buffer exchange applications with samples up to 2 ml. For optimal performance with very dilute samples, e.g. forensic specimens, Vivacon® 2 is equipped with the patented Hydrosart® regenerated cellulose membrane that guarantees extremely low binding properties and high flux. High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-off printed on individual devices.
Vivacon® 2 features a horizontal membrane design that is preferred by some users for reasons of consistency with previously used procedures and high recovery. The possibility of a back-spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.
- Sterile and free of DNA*
- Convenient handling in fixed-angle rotors
- Low binding material
- Back-spin possibility
- Graduation and MWCO printed on
- Complete and highly reproducible concentrate recovery
qPCR assay over 55 cycles with a verified limit of detection of < 2 pg.
For smaller volumes of 500 µl (0.5 ml) and below Vivacon® 500 ETO with identical features is available.
Vivacon® 2 Applications
- DNA rebuffering of organic extraction samples before sequencing reaction.
- Dye removal after sequencing reaction.
- Primer removal.
- Plasmid concentration.
Performance Characteristics - Vivacon® 2 for DNA
|
Sample size (bp) |
Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) |
|
| 2,000 MWCO | 10 | 120 min | 92 % | 7,500 |
| 10,000 MWCO | 30 | 60 min | 94 % | 5,000 |
| 30,000 MWCO | 50 | 60 min | 95 % | 2,500 |
| 50,000 MWCO | 300 | 45 min | 96 % | 2,500 |
| 100,000 MWCO | 600 | 30 min | 93 % | 2,500 |
| 125,000 MWCO | 650 | 30 min | 88 % | 2,000 |
| 125,000 MWCO | 900 | 30 min | 89 % | 2,000 |
Start volume 2.0 ml, sample concentration 50 ng/ml
Performance Characteristics - Vivacon® 2 for proteins
| Sample |
Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) |
|
| 2,000 MWCO | 0.25 mg/ml cytochrome c | 120 min | 95 % | 7,500 |
| 10,000 MWCO | 0.25 mg/ml cytochrome c | 90 min | 96 % | 5,000 |
| 30,000 MWCO | 1.0 mg/ml BSA | 40 min | 96 % | 5,000 |
| 50,000 MWCO | 1.0 mg/ml BSA | 30 min | 94 % | 5,000 |
| 100,000 MWCO | 1.0 mg/ml bovine lgG | 30 min | 92 % | 5,000 |
| 125,000 MWCO | 1.0 mg/ml bovine lgG | 27 min | 81 % | 5,000 |
Start volume 2.0 ml, sample and concentration of protein as specified in table
Conversion Table for Hydrosart® MWCO to Nucleotide Cut-off
| Membrane | MWCO |
Double-Stranded Nucleotide Cut-off (bp) |
| Hydrosart® | 2 kDa | > 10 |
| Hydrosart® | 10 kDa | > 30 |
| Hydrosart® | 30 kDa | > 50 |
| Hydrosart® | 50 kDa | > 300 |
| Hydrosart® | 100 kDa | > 600 |
| Cellulose Triacetate | 125 kDa | > 650 |
| Feature | Benefit |
| Re-spin possibility | Complete and highly reproducible sample recovery |
| Low binding material | High recoveries of low sample recovery |
| Easy to remove re-spin cap | Convenient sample handling |
| Graduation printed on | Optimal process control |
Tech Specs
| Concentrator Capacity | |
| Fixed Angle Rotor | 2 ml |
| Dimensions | |
| Total Length | 125 mm (concentration) |
| Total Length | 115 mm (backspin) |
| Width | 16 mm |
| Active Membrane Area | 0.95 cm² |
| Hold-up volume membrane and support | 10 µl |
| Dead stop volume | 55 µl (25° rotor) |
| Materials of Construction | |
| Body | Polycarbonate |
| Filtrate vessel | Polypropylene |
| Back spin vial | Polypropylene |
| Concentrator Cap | Polypropylene |
| O Ring | Silicone |
| Membrane (ex 125,000 MWCO) | Hydrosart® |
| Membrane (125,000 MWCO) | Cellulose Triacetate |
| Equipment Required - Centrifuge | |
| Rotor type | Fixed Angle |
| Minimum rotor angle | 25° |
| Rotor cavity | To fit 15 ml (17 mm) conical bottom tubes (95 mm depth) |
| Maximum speed |
2,500 g (May be increased in some cases. See manual for details). Also note that DNA recovery may improve at lower g forces. |
Ordering Information
| Description | Prod. No. | Price | |
| PCR Grade, 100,000 MWCO, Hydrosart Membrane, 100 per pack | VN02H42ETO |
$734.00 |
Add to Cart |
|
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN02H43ETO |
$3,100.00 |
Add to Cart |