Vivacon 2 ETO

Vivacon® 2 ETO

PCR Grade Vivacons achieved by Ethylene Oxide Treatment

The new Vivacon® 2 PCR Grade units, in addition to all features described below for Vivacon® 2, have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 10-6 and free of detectable human genomic DNA contamination tested with a qPCR assay over 55 cycles with a verified limit of detection of < 2 pg.

Vivacon® 2 are centrifugal concentrators for DNA concentration and buffer exchange applications with samples up to 2 ml. For optimal performance with very dilute samples, e.g. forensic specimens, Vivacon® 2 is equipped with the patented Hydrosart® regenerated cellulose membrane that guarantees extremely low binding properties and high flux. High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-off printed on individual devices.

Vivacon® 2 features a horizontal membrane design that is preferred by some users for reasons of consistency with previously used procedures and high recovery. The possibility of a back-spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.

  • Sterile and free of DNA*
  • Convenient handling in fixed-angle rotors
  • Low binding material
  • Back-spin possibility
  • Graduation and MWCO printed on
  • Complete and highly reproducible concentrate recovery

qPCR assay over 55 cycles with a verified limit of detection of < 2 pg.

For smaller volumes of 500 µl (0.5 ml) and below Vivacon® 500 ETO with identical features is available.

Vivacon® 2 Applications

  • DNA rebuffering of organic extraction samples before sequencing reaction.
  • Dye removal after sequencing reaction.
  • Primer removal.
  • Plasmid concentration.

Performance Characteristics - Vivacon® 2 for DNA

Sample size
(bp)
Time to concentrate
up to 30x [min.]
at 20°C
Concentrate
recovery %
g-force
(xg)
2,000 MWCO 10 120 min 92 % 7,500
10,000 MWCO 30 60 min 94 % 5,000
30,000 MWCO 50 60 min 95 % 2,500
50,000 MWCO 300 45 min 96 % 2,500
100,000 MWCO 600 30 min 93 % 2,500
125,000 MWCO 650 30 min 88 % 2,000
125,000 MWCO 900 30 min 89 % 2,000

Start volume 2.0 ml, sample concentration 50 ng/ml

Performance Characteristics - Vivacon® 2 for proteins

Sample Time to concentrate
up to 30x [min.]
at 20°C
Concentrate
recovery %
g-force
(xg)
2,000 MWCO 0.25 mg/ml cytochrome c 120 min 95 % 7,500
10,000 MWCO 0.25 mg/ml cytochrome c 90 min 96 % 5,000
30,000 MWCO 1.0 mg/ml BSA 40 min 96 % 5,000
50,000 MWCO 1.0 mg/ml BSA 30 min 94 % 5,000
100,000 MWCO 1.0 mg/ml bovine lgG 30 min 92 % 5,000
125,000 MWCO 1.0 mg/ml bovine lgG 27 min 81 % 5,000

Start volume 2.0 ml, sample and concentration of protein as specified in table

Conversion Table for Hydrosart® MWCO to Nucleotide Cut-off

Membrane MWCO Double-Stranded
Nucleotide Cut-off
(bp)
Hydrosart® 2 kDa > 10
Hydrosart® 10 kDa > 30
Hydrosart® 30 kDa > 50
Hydrosart® 50 kDa > 300
Hydrosart® 100 kDa > 600
Cellulose Triacetate 125 kDa > 650
Feature Benefit
Re-spin possibility Complete and highly reproducible sample recovery
Low binding material High recoveries of low sample recovery
Easy to remove re-spin cap Convenient sample handling
Graduation printed on Optimal process control

Tech Specs

Concentrator Capacity
Fixed Angle Rotor 2 ml
Dimensions
Total Length 125 mm (concentration)
Total Length 115 mm (backspin)
Width 16 mm
Active Membrane Area 0.95 cm²
Hold-up volume membrane and support 10 µl
Dead stop volume 55 µl (25° rotor)
Materials of Construction
Body Polycarbonate
Filtrate vessel Polypropylene
Back spin vial Polypropylene
Concentrator Cap Polypropylene
O Ring Silicone
Membrane (ex 125,000 MWCO) Hydrosart®
Membrane (125,000 MWCO) Cellulose Triacetate
Equipment Required - Centrifuge
Rotor type Fixed Angle
Minimum rotor angle 25°
Rotor cavity To fit 15 ml (17 mm) conical bottom tubes (95 mm depth)
Maximum speed 2,500 g (May be increased in some cases. See manual for details).
Also note that DNA recovery may improve at lower g forces.

Ordering Information

Description Prod. No. Price
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 25 per pack VN02H41ETO $177.00
Add to Cart
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 100 per pack VN02H42ETO $635.00
Add to Cart
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 500 per pack
 
For larger quantities - please contact us for a quotation.
VN02H43ETO $2,700.00
Add to Cart

Accessories Ordering Information

Description Prod. No. Price
VIVACON 2 RACK VNA001 $31.00
Add to Cart
Copyright © Vivaproducts 1998-2019.
Website Created by Intrepid Web Solutions