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Vivacon® 2 ETO Centrifugal Concentrator

Up to 2 ml starting volume
Concentrate up to 30x

Vivacon 2 ETO devices allow rapid concentration of DNA and PCR primer removal for samples up to 2 ml volume. Vivacon 2 ETO uses low binding Hydrosart® regenerated cellulose membranes positioned horizontally for optimal results in these applications. They can be used in centrifuges with fixed angle rotors accepting 15 ml centrifuge tubes. The Vivacon 2 ETO also allows reverse spinning of your sample into the upper recovery cap for simplified recovery. They have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 106 and free of detectable human genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg.

Data Sheet

Technical Data and Operating Instructions

Ethylene oxide treatment as a method for introducing PCR grade Vivacon® centrifugal concentrators

Primer removal after a PCR reaction with Vivacon® 2

ETO Vivacon Concordance Study

DNA rebuffering of organic extraction samples before sequencing reaction.
Dye removal after sequencing reaction.
Primer removal.
Plasmid concentration.

Vivacon® 2 for DNA

	Sample size (bp)	Time to concentrate up to 30x [min.] at 20°C	Concentrate recovery %	g-force (xg)
2,000 MWCO	10	120 min	92 %	7,500
10,000 MWCO	30	60 min	94 %	5,000
30,000 MWCO	50	60 min	95 %	2,500
50,000 MWCO	300	45 min	96 %	2,500
100,000 MWCO	600	30 min	93 %	2,500
125,000 MWCO	650	30 min	88 %	2,000
125,000 MWCO	900	30 min	89 %	2,000

Start volume 2.0 ml, sample concentration 50 ng/ml

Vivacon® 2 for proteins

	Sample	Time to concentrate up to 30x [min.] at 20°C	Concentrate recovery %	g-force (xg)
2,000 MWCO	0.25 mg/ml cytochrome c	120 min	95 %	7,500
10,000 MWCO	0.25 mg/ml cytochrome c	90 min	96 %	5,000
30,000 MWCO	1.0 mg/ml BSA	40 min	96 %	5,000
50,000 MWCO	1.0 mg/ml BSA	30 min	94 %	5,000
100,000 MWCO	1.0 mg/ml bovine lgG	30 min	92 %	5,000
125,000 MWCO	1.0 mg/ml bovine lgG	27 min	81 %	5,000

Start volume 2.0 ml, sample and concentration of protein as specified in table

Membrane	MWCO	Double-Stranded Nucleotide Cut-off (bp)
Hydrosart®	2 kDa	> 10
Hydrosart®	10 kDa	> 30
Hydrosart®	30 kDa	> 50
Hydrosart®	50 kDa	> 300
Hydrosart®	100 kDa	> 600
Cellulose Triacetate	125 kDa	> 650

Concentrator Capacity
Fixed Angle Rotor	2 ml
Dimensions
Total Length	125 mm (concentration)
Total Length	115 mm (backspin)
Width	16 mm
Active Membrane Area	0.95 cm²
Hold-up volume, membrane and support	<10 µl
Dead stop volume (fixed angle rotor*)	55 µl (25° rotor)
Dead stop volume (swing bucket rotor*)	No dead stop
Materials of Construction
Body	Polycarbonate
Filtrate Vessel	Polypropylene
Back Spin Vial	Polypropylene
Concentrator Cap	Polypropylene
O Ring	Silicone
Membrane (ex 125,000 MWCO)	Hydrosart®
Membrane (125,000 MWCO)	Cellulose Triacetate
Equipment Required - Centrifuge
Rotor type	Fixed Angle
Rotor Cavity	To fit 15 ml (17 mm) conical bottom tubes (95 mm depth)
Maximum speed	2,500x g (May be increased in some cases. See manual for details). Also note that DNA recovery may improve at lower g forces.

Description	Prod. #	Price	Buy
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 100 per pack SKU: VN02H42ETO Price: $775.00	VN02H42ETO	$775.00	Add to Cart
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 500 per pack SKU: VN02H43ETO Price: $3,300.00	VN02H43ETO	$3,300.00	Add to Cart