PCR Grade Vivacons achieved by Ethylene Oxide Treatment
The new Vivacon® 2 PCR Grade units, in addition to all features described below for Vivacon® 2, have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 10-6 and free of detectable human genomic DNA contamination tested with a qPCR assay over 55 cycles with a verified limit of detection of < 2 pg.
Vivacon® 2 are centrifugal concentrators for DNA concentration and buffer exchange applications with samples up to 2 ml. For optimal performance with very dilute samples, e.g. forensic specimens, Vivacon® 2 is equipped with the patented Hydrosart® regenerated cellulose membrane that guarantees extremely low binding properties and high flux. High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-off printed on individual devices.
Vivacon® 2 features a horizontal membrane design that is preferred by some users for reasons of consistency with previously used procedures and high recovery. The possibility of a back-spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.
qPCR assay over 55 cycles with a verified limit of detection of < 2 pg.
For smaller volumes of 500 µl (0.5 ml) and below Vivacon® 500 ETO with identical features is available.
Sample size (bp) |
Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) |
|
2,000 MWCO | 10 | 120 min | 92 % | 7,500 |
10,000 MWCO | 30 | 60 min | 94 % | 5,000 |
30,000 MWCO | 50 | 60 min | 95 % | 2,500 |
50,000 MWCO | 300 | 45 min | 96 % | 2,500 |
100,000 MWCO | 600 | 30 min | 93 % | 2,500 |
125,000 MWCO | 650 | 30 min | 88 % | 2,000 |
125,000 MWCO | 900 | 30 min | 89 % | 2,000 |
Start volume 2.0 ml, sample concentration 50 ng/ml
Sample |
Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) |
|
2,000 MWCO | 0.25 mg/ml cytochrome c | 120 min | 95 % | 7,500 |
10,000 MWCO | 0.25 mg/ml cytochrome c | 90 min | 96 % | 5,000 |
30,000 MWCO | 1.0 mg/ml BSA | 40 min | 96 % | 5,000 |
50,000 MWCO | 1.0 mg/ml BSA | 30 min | 94 % | 5,000 |
100,000 MWCO | 1.0 mg/ml bovine lgG | 30 min | 92 % | 5,000 |
125,000 MWCO | 1.0 mg/ml bovine lgG | 27 min | 81 % | 5,000 |
Start volume 2.0 ml, sample and concentration of protein as specified in table
Membrane | MWCO |
Double-Stranded Nucleotide Cut-off (bp) |
Hydrosart® | 2 kDa | > 10 |
Hydrosart® | 10 kDa | > 30 |
Hydrosart® | 30 kDa | > 50 |
Hydrosart® | 50 kDa | > 300 |
Hydrosart® | 100 kDa | > 600 |
Cellulose Triacetate | 125 kDa | > 650 |
Feature | Benefit |
Re-spin possibility | Complete and highly reproducible sample recovery |
Low binding material | High recoveries of low sample recovery |
Easy to remove re-spin cap | Convenient sample handling |
Graduation printed on | Optimal process control |
Concentrator Capacity | |
Fixed Angle Rotor | 2 ml |
Dimensions | |
Total Length | 125 mm (concentration) |
Total Length | 115 mm (backspin) |
Width | 16 mm |
Active Membrane Area | 0.95 cm² |
Hold-up volume membrane and support | 10 µl |
Dead stop volume | 55 µl (25° rotor) |
Materials of Construction | |
Body | Polycarbonate |
Filtrate vessel | Polypropylene |
Back spin vial | Polypropylene |
Concentrator Cap | Polypropylene |
O Ring | Silicone |
Membrane (ex 125,000 MWCO) | Hydrosart® |
Membrane (125,000 MWCO) | Cellulose Triacetate |
Equipment Required - Centrifuge | |
Rotor type | Fixed Angle |
Minimum rotor angle | 25° |
Rotor cavity | To fit 15 ml (17 mm) conical bottom tubes (95 mm depth) |
Maximum speed |
2,500 g (May be increased in some cases. See manual for details). Also note that DNA recovery may improve at lower g forces. |
Description | Prod. No. | Price | |
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 25 per pack | VN02H41ETO |
$185.00 |
Add to Cart |
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 100 per pack | VN02H42ETO |
$660.00 |
Add to Cart |
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN02H43ETO |
$2,800.00 |
Add to Cart |
Description | Prod. No. | Price | |
VIVACON 2 RACK | VNA001 |
$30.00 |
Add to Cart |