Vivacon 2

Vivacon® 2

Reproducible DNA sample desalting and concentration

Vivacon® 2 centrifugal concentrators offer the optimal solution for DNA concentration and buffer exchange applications. For optimal performance with very dilute samples, e. g. forensic samples, Vivacon® 2 is equipped with the patented regenerated cellulose membrane Hydrosart®. High recoveries and excellent reproducibilities are paired with convenience offered by volume graduation and molecular weight cut-off printed on individual devices.

The possibility of a re-spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.

Vivacon® 2 Applications

  • DNA rebuffering of organic extraction samples before sequencing reaction.
  • Dye removal after sequencing reaction.
  • Primer removal.
  • Plasmid concentration.

Performance Characteristics - Vivacon® 2 for DNA

Sample size
(bp)
Time to concentrate
up to 30x [min.]
at 20°C
Concentrate
recovery %
g-force
(xg)
2,000 MWCO 10 120 min 92 % 7,500
10,000 MWCO 30 60 min 94 % 5,000
30,000 MWCO 50 60 min 95 % 2,500
50,000 MWCO 300 45 min 96 % 2,500
100,000 MWCO 600 30 min 93 % 2,500
125,000 MWCO 650 30 min 88 % 2,000
125,000 MWCO 900 30 min 89 % 2,000

Start volume 2.0 ml, sample concentration 50 ng/ml

Performance Characteristics - Vivacon® 2 for proteins

Sample Time to concentrate
up to 30x [min.]
at 20°C
Concentrate
recovery %
g-force
(xg)
2,000 MWCO 0.25 mg/ml cytochrome c 120 min 95 % 7,500
10,000 MWCO 0.25 mg/ml cytochrome c 90 min 96 % 5,000
30,000 MWCO 1.0 mg/ml BSA 40 min 96 % 5,000
50,000 MWCO 1.0 mg/ml BSA 30 min 94 % 5,000
100,000 MWCO 1.0 mg/ml bovine lgG 30 min 92 % 5,000
125,000 MWCO 1.0 mg/ml bovine lgG 27 min 81 % 5,000

Start volume 2.0 ml, sample and concentration of protein as specified in table

Conversion Table for Hydrosart® MWCO to Nucleotide Cut-off

Membrane MWCO Double-Stranded
Nucleotide Cut-off
(bp)
Hydrosart® 2 kDa > 10
Hydrosart® 10 kDa > 30
Hydrosart® 30 kDa > 50
Hydrosart® 50 kDa > 300
Hydrosart® 100 kDa > 600
Cellulose Triacetate 125 kDa > 650
Feature Benefit
Re-spin possibility Complete and highly reproducible sample recovery
Low binding material High recoveries of low sample recovery
Easy to remove re-spin cap Convenient sample handling
Graduation printed on Optimal process control

Tech Specs

Concentrator Capacity
Fixed Angle Rotor 2 ml
Dimensions
Total Length 125 mm (concentration)
Total Length 115 mm (backspin)
Width 16 mm
Active Membrane Area 0.95 cm²
Hold-up volume membrane and support 10 µl
Dead stop volume 55 µl (25° rotor)
Materials of Construction
Body Polycarbonate
Filtrate vessel Polypropylene
Back spin vial Polypropylene
Concentrator Cap Polypropylene
O Ring Silicone
Membrane (ex 125,000 MWCO) Hydrosart®
Membrane (125,000 MWCO) Cellulose Triacetate
Equipment Required - Centrifuge
Rotor type Fixed Angle
Minimum rotor angle 25°
Rotor cavity To fit 15 ml (17 mm) conical bottom tubes (95 mm depth)
Maximum speed 2,500 g (May be increased in some cases. See manual for details).
Also note that DNA recovery may improve at lower g forces.

Ordering Information

Description Prod. No. Price
2,000 MWCO, Hydrosart Membrane, 25 per pack VN02H91 $158.00
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2,000 MWCO, Hydrosart Membrane, 100 per pack VN02H92 $565.00
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10,000 MWCO, Hydrosart Membrane, 25 per pack VN02H01 $158.00
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10,000 MWCO, Hydrosart Membrane, 100 per pack VN02H02 $565.00
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30,000 MWCO, Hydrosart Membrane, 25 per pack VN02H21 $158.00
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30,000 MWCO, Hydrosart Membrane, 100 per pack VN02H22 $565.00
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30,000 MWCO, Hydrosart Membrane, 500 per pack
 
For larger quantities - please contact us for a quotation.
VN02H23 $2,495.00
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50,000 MWCO, Hydrosart Membrane, 25 per pack VN02H31 $158.00
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50,000 MWCO, Hydrosart Membrane, 100 per pack VN02H32 $565.00
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50,000 MWCO, Hydrosart Membrane, 500 per pack
 
For larger quantities - please contact us for a quotation.
VN02H33 $2,495.00
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100,000 MWCO, Hydrosart Membrane, 25 per pack VN02H41 $158.00
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100,000 MWCO, Hydrosart Membrane, 100 per pack VN02H42 $565.00
Add to Cart
100,000 MWCO, Hydrosart Membrane, 500 per pack
 
For larger quantities - please contact us for a quotation.
VN02H43 $2,495.00
Add to Cart
125,000 MWCO, Cellulose Triacetate Membrane, 25 per pack
 
Special order only - please contact us for a quotation.
VN02H81 $158.00
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125,000 MWCO, Cellulose Triacetate Membrane, 100 per pack
 
Special order only - please contact us for a quotation.
VN02H82 $565.00
Contact Us
125,000 MWCO, Cellulose Triacetate Membrane, 500 per pack
 
Special order only - please contact us for a quotation.
VN02H83 $2,495.00
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Accessories Ordering Information

Description Prod. No. Price
VIVACON 2 RACK VNA001 $31.00
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