Vivacon 500 ETO

Vivacon® 500 ETO

PCR Grade Vivacons achieved by Ethylene Oxide Treatment

The new Vivacon® 500 PCR Grade units, in addition to all features described for Vivacon® 500, have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 10-6 and free of detectable human genomic DNA contamination tested by using a qPCR assay over 55 cycles with a verified limit of detection < 2 pg.

Vivacon® 500 are centrifugal concentrators for the concentration of DNA or proteins and buffer exchange applications with samples up to 500 µl. For optimal performance with very dilute samples, e.g. forensic specimens, Vivacon® 500 is equipped with the patented Hydrosart® regenerated cellulose membrane that guarantees extremely low binding properties and high flux. High recoveries and excellent reproducibility are paired with convenience offered by the molecular weight cut-off printed on individual devices.

Vivacon® 500 features a horizontal membrane design that is preferred by some users for reasons of consistency with previously used procedures and high reproducibility. The possibility of a back-spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.

  • Sterile and free of DNA*
  • Convenient handling in fixed-angle rotors
  • Low binding material
  • Back-spin possibility
  • MWCO printed on
  • Complete and highly reproducible concentrate recovery

* qPCR assay over 55 cycles with a verified limit of detection < 2 pg.

For larger volumes up to 2 ml Vivacon® 2 ETO with identical features is available.

Applications

  • DNA rebuffering of organic extraction samples before sequencing reaction.
  • Dye removal after sequencing reaction.
  • Primer removal.
  • Plasmid concentration.

Performance Characteristics - Vivacon® 500 for DNA

Sample size
(bp)
Time to concentrate
up to 30x [min.]
at 20°C
Concentrate
recovery %
g-force
(xg)
2,000 MWCO 10 60 min 93 % 7,500
10,000 MWCO 30 25 min 94 % 7,500
30,000 MWCO 50 18 min 88 % 5,000
50,000 MWCO 300 18 min 91 % 3,000
100,000 MWCO 600 10 min 87 % 3,000
125,000 MWCO 650 12 min 85 % 2,000
125,000 MWCO 900 9 min 94 % 3,000

Start volume 0.5 ml, sample concentration 50 ng/ml

Performance Characteristics - Vivacon® 500 for proteins

Sample Time to concentrate
up to 30x [min.]
at 20°C
Concentrate
recovery %
g-force
(xg)
2,000 MWCO 0.25 mg/ml cytochrome c 30 min 95 % 14,000
10,000 MWCO 0.25 mg/ml cytochrome c 15 min 92 % 14,000
30,000 MWCO 1.0 mg/ml BSA 10 min 95 % 14,000
50,000 MWCO 1.0 mg/ml BSA 10 min 92 % 14,000
100,000 MWCO 1.0 mg/ml bovine lgG 11 min 90 % 8,000
100,000 MWCO 0.02 mg/ml porcine hematin 10 min * 88 % 2,500
125,000 MWCO 1.0 mg/ml bovine lgG 10 min 81 % 8,000
125,000 MWCO 0.02 mg/ml porcine hematin 10 min * 11 % 2,500

Start volume 0.5 ml, sample and concentration of protein as specified in table

* Note that the 10 minute spin for the hematin samples gave a concentration factor of 6x for the 100,000 MWCO and 75x for the 125,000 MWCO

Conversion Table for Hydrosart® MWCO to Nucleotide Cut-off

Membrane MWCO Double-Stranded
Nucleotide Cut-off
(bp)
Hydrosart® 2 kDa > 10
Hydrosart® 10 kDa > 30
Hydrosart® 30 kDa > 50
Hydrosart® 50 kDa > 300
Hydrosart® 100 kDa > 600
Cellulose Triacetate 125 kDa > 650

Features & Benefits

Feature Benefit
Low binding material High recoveries of low sample recovery
Re-spin possibility Complete and highly reproducible sample recovery

Tech Specs

Concentrator Capacity
Fixed Angle Rotor 0.5 ml
Dimensions
Total Length 45 mm (concentration)
Total Length 47.5 mm (backspin)
Width 12.4 mm
Active Membrane Area 0.32 cm²
Hold-up volume membrane and support < 5 µl
Dead stop volume 5 µl (40° rotor)
Materials of Construction
Body Polycarbonate
Filtrate vessel Polypropylene
Back spin vial Polypropylene
O Ring Silicone
Membrane (ex 125,000 MWCO) Hydrosart®
Membrane (125,000 MWCO) Cellulose Triacetate
Equipment Required - Centrifuge
Rotor type Fixed angle
Minimum rotor angle 40°
Rotor cavity To fit 1.5 | 2.2 ml (11 mm) conical bottom tubes
Maximum speed 5,000 g (May be increased in some cases. See manual for details).
Also note that DNA recovery may improve at lower g forces.

Ordering Information

Description Prod. No. Price
PCR Grade, 30,000 MWCO, Hydrosart Membrane, 25 per pack VN01H21ETO $147.00
Add to Cart
PCR Grade, 30,000 MWCO, Hydrosart Membrane, 100 per pack VN01H22ETO $480.00
Add to Cart
PCR Grade, 30,000 MWCO, Hydrosart Membrane, 500 per pack
 
For larger quantities - please contact us for a quotation.
VN01H23ETO $2,130.00
Add to Cart
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 25 per pack VN01H41ETO $147.00
Add to Cart
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 100 per pack VN01H42ETO $480.00
Add to Cart
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 500 per pack
 
For larger quantities - please contact us for a quotation.
VN01H43ETO $2,130.00
Add to Cart

Accessories Ordering Information


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Description Prod. No. Price
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