Vivacon® 500 ETO
PCR Grade Vivacons achieved by Ethylene Oxide Treatment
The new Vivacon® 500 PCR Grade units, in addition to all features described for Vivacon® 500, have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 10-6 and free of detectable human genomic DNA contamination tested by using a qPCR assay over 55 cycles with a verified limit of detection < 2 pg.
Vivacon® 500 are centrifugal concentrators for the concentration of DNA or proteins and buffer exchange applications with samples up to 500 µl. For optimal performance with very dilute samples, e.g. forensic specimens, Vivacon® 500 is equipped with the patented Hydrosart® regenerated cellulose membrane that guarantees extremely low binding properties and high flux. High recoveries and excellent reproducibility are paired with convenience offered by the molecular weight cut-off printed on individual devices.
Vivacon® 500 features a horizontal membrane design that is preferred by some users for reasons of consistency with previously used procedures and high reproducibility. The possibility of a back-spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.
- Sterile and free of DNA*
- Convenient handling in fixed-angle rotors
- Low binding material
- Back-spin possibility
- MWCO printed on
- Complete and highly reproducible concentrate recovery
* qPCR assay over 55 cycles with a verified limit of detection < 2 pg.
For larger volumes up to 2 ml Vivacon® 2 ETO with identical features is available.
Applications
- DNA rebuffering of organic extraction samples before sequencing reaction.
- Dye removal after sequencing reaction.
- Primer removal.
- Plasmid concentration.
Performance Characteristics - Vivacon® 500 for DNA
|
Sample size (bp) |
Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) |
|
| 2,000 MWCO | 10 | 60 min | 93 % | 7,500 |
| 10,000 MWCO | 30 | 25 min | 94 % | 7,500 |
| 30,000 MWCO | 50 | 18 min | 88 % | 5,000 |
| 50,000 MWCO | 300 | 18 min | 91 % | 3,000 |
| 100,000 MWCO | 600 | 10 min | 87 % | 3,000 |
| 125,000 MWCO | 650 | 12 min | 85 % | 2,000 |
| 125,000 MWCO | 900 | 9 min | 94 % | 3,000 |
Start volume 0.5 ml, sample concentration 50 ng/ml
Performance Characteristics - Vivacon® 500 for proteins
| Sample |
Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) |
|
| 2,000 MWCO | 0.25 mg/ml cytochrome c | 30 min | 95 % | 14,000 |
| 10,000 MWCO | 0.25 mg/ml cytochrome c | 15 min | 92 % | 14,000 |
| 30,000 MWCO | 1.0 mg/ml BSA | 10 min | 95 % | 14,000 |
| 50,000 MWCO | 1.0 mg/ml BSA | 10 min | 92 % | 14,000 |
| 100,000 MWCO | 1.0 mg/ml bovine lgG | 11 min | 90 % | 8,000 |
| 100,000 MWCO | 0.02 mg/ml porcine hematin | 10 min * | 88 % | 2,500 |
| 125,000 MWCO | 1.0 mg/ml bovine lgG | 10 min | 81 % | 8,000 |
| 125,000 MWCO | 0.02 mg/ml porcine hematin | 10 min * | 11 % | 2,500 |
Start volume 0.5 ml, sample and concentration of protein as specified in table
* Note that the 10 minute spin for the hematin samples gave a concentration factor of 6x for the 100,000 MWCO and 75x for the 125,000 MWCO
Conversion Table for Hydrosart® MWCO to Nucleotide Cut-off
| Membrane | MWCO |
Double-Stranded Nucleotide Cut-off (bp) |
| Hydrosart® | 2 kDa | > 10 |
| Hydrosart® | 10 kDa | > 30 |
| Hydrosart® | 30 kDa | > 50 |
| Hydrosart® | 50 kDa | > 300 |
| Hydrosart® | 100 kDa | > 600 |
| Cellulose Triacetate | 125 kDa | > 650 |
Features & Benefits
| Feature | Benefit | Low binding material | High recoveries of low sample recovery |
| Re-spin possibility | Complete and highly reproducible sample recovery |
Tech Specs
| Concentrator Capacity | |
| Fixed Angle Rotor | 0.5 ml |
| Dimensions | |
| Total Length | 45 mm (concentration) |
| Total Length | 47.5 mm (backspin) |
| Width | 12.4 mm |
| Active Membrane Area | 0.32 cm² |
| Hold-up volume membrane and support | < 5 µl |
| Dead stop volume | 5 µl (40° rotor) |
| Materials of Construction | |
| Body | Polycarbonate |
| Filtrate vessel | Polypropylene |
| Back spin vial | Polypropylene |
| O Ring | Silicone |
| Membrane (ex 125,000 MWCO) | Hydrosart® |
| Membrane (125,000 MWCO) | Cellulose Triacetate |
| Equipment Required - Centrifuge | |
| Rotor type | Fixed angle |
| Minimum rotor angle | 40° |
| Rotor cavity | To fit 1.5 | 2.2 ml (11 mm) conical bottom tubes |
| Maximum speed |
5,000 g (May be increased in some cases. See manual for details). Also note that DNA recovery may improve at lower g forces. |
Ordering Information
| Description | Prod. No. | Price | |
| PCR Grade, 30,000 MWCO, Hydrosart Membrane, 100 per pack | VN01H22ETO |
$553.00 |
Add to Cart |
|
PCR Grade, 30,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN01H23ETO |
$2,430.00 |
Add to Cart |
| PCR Grade, 100,000 MWCO, Hydrosart Membrane, 100 per pack | VN01H42ETO |
$553.00 |
Add to Cart |
|
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN01H43ETO |
$2,430.00 |
Add to Cart |