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Vivacon® 500 ETO Centrifugal Concentrator

Up to 500 µl starting volume
Concentrate up to 50x

Vivacon 500 ETO devices allow rapid concentration of DNA and PCR primer removal for samples up to 500 µl volume. Vivacon 500 ETO uses low binding Hydrosart® regenerated cellulose membranes positioned horizontally for optimal results in these applications. They can be used in micro-centrifuges with fixed angle rotors accepting 2.2 ml centrifuge tubes. The Vivacon 500 ETO also allows reverse spinning of your sample for simplified recovery. They have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 106 and free of detectable human genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg.

Data Sheet

Technical Data and Operating Instructions

Ethylene oxide treatment as a method for introducing PCR grade Vivacon® centrifugal concentrators

Primer removal after a PCR reaction with Vivacon® 500

ETO Vivacon Concordance Study

DNA rebuffering of organic extraction samples before sequencing reaction.
Dye removal after sequencing reaction.
Primer removal.
Plasmid concentration.

Vivacon® 500 for DNA

	Sample size (bp)	Time to concentrate up to 30x [min.] at 20°C	Concentrate recovery %	g-force (xg)
2,000 MWCO	10	60 min	93 %	7,500
10,000 MWCO	30	25 min	94 %	7,500
30,000 MWCO	50	18 min	88 %	5,000
50,000 MWCO	300	18 min	91 %	3,000
100,000 MWCO	600	10 min	87 %	3,000
125,000 MWCO	650	12 min	85 %	2,000
125,000 MWCO	900	9 min	94 %	3,000

Start volume 0.5 ml, sample concentration 50 ng/ml

Vivacon® 500 for proteins

	Sample	Time to concentrate up to 30x [min.] at 20°C	Concentrate recovery %	g-force (xg)
2,000 MWCO	0.25 mg/ml cytochrome c	30 min	95 %	14,000
10,000 MWCO	0.25 mg/ml cytochrome c	15 min	92 %	14,000
30,000 MWCO	1.0 mg/ml BSA	10 min	95 %	14,000
50,000 MWCO	1.0 mg/ml BSA	10 min	92 %	14,000
100,000 MWCO	1.0 mg/ml bovine lgG	11 min	90 %	8,000
100,000 MWCO	0.02 mg/ml porcine hematin	10 min ^✝	88 %	2,500
125,000 MWCO	1.0 mg/ml bovine lgG	10 min	81 %	8,000
125,000 MWCO	0.02 mg/ml porcine hematin	10 min ^✝	11 %	2,500

Start volume 0.5 ml, sample and concentration of protein as specified in table

^✝ Note that the 10 minute spin for the hematin samples gave a concentration factor of 6x for the 100,000 MWCO and 75x for the 125,000 MWCO

Membrane	MWCO	Double-Stranded Nucleotide Cut-off (bp)
Hydrosart®	2 kDa	> 10
Hydrosart®	10 kDa	> 30
Hydrosart®	30 kDa	> 50
Hydrosart®	50 kDa	> 300
Hydrosart®	100 kDa	> 600
Cellulose Triacetate	125 kDa	> 650

Concentrator Capacity
Fixed Angle Rotor	0.5 ml
Dimensions
Total Length	45 mm (concentration)
Total Length	47.5 mm (backspin)
Width	12.4 mm
Active Membrane Area	0.32 cm²
Hold-up volume, membrane and support	<5 µl
Dead stop volume	5 µl (40° rotor)
Materials of Construction
Body	Polycarbonate
Filtrate Vessel	Polypropylene
Back Spin Vial	Polypropylene
O Ring	Silicone
Membrane (ex 125,000 MWCO)	Hydrosart®
Membrane (125,000 MWCO)	Cellulose Triacetate
Equipment Required - Centrifuge
Rotor type	Fixed Angle
Minimum rotor angle	40°
Rotor Cavity	To fit 1.5 \| 2.2 ml (11 mm) conical bottom tubes (95 mm depth)
Maximum speed	5,000x g (May be increased in some cases. See manual for details). Also note that DNA recovery may improve at lower g forces.

Description	Prod. #	Price	Buy
PCR Grade, 30,000 MWCO, Hydrosart Membrane, 100 per pack SKU: VN01H22ETO Price: $583.00	VN01H22ETO	$583.00	Add to Cart
PCR Grade, 30,000 MWCO, Hydrosart Membrane, 500 per pack SKU: VN01H23ETO Price: $2,600.00	VN01H23ETO	$2,600.00	Add to Cart
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 100 per pack SKU: VN01H42ETO Price: $583.00	VN01H42ETO	$583.00	Add to Cart
PCR Grade, 100,000 MWCO, Hydrosart Membrane, 500 per pack SKU: VN01H43ETO Price: $2,600.00	VN01H43ETO	$2,600.00	Add to Cart