Vivacon® 500

Vivacon® 500

Reproducible DNA sample desalting and concentration

Vivacon® 500 centrifugal concentrators offer the optimal solution for DNA concentration and buffer exchange applications. For optimal performance with very dilute samples, e. g. forensic samples, Vivacon® 500 is equipped with the patented regenerated cellulose membrane Hydrosart®. High recoveries and excellent reproducibilities are paired with convenience offered by volume graduation and molecular weight cut-off printed on individual devices.

Vivacon® 500 can be used as well for concentration and buffer exchange of protein samples.

Vivacon® 500 Applications

  • DNA rebuffering of organic extraction samples before sequencing reaction
  • Dye removal after sequencing reaction
  • Primer removal
  • Plasmid concentration
  • Protein concentration
  • Protein rebuffering

For larger volumes up to 2 ml Vivacon® 2 with identical features is available.

Applications

  • DNA rebuffering of organic extraction samples before sequencing reaction.
  • Dye removal after sequencing reaction.
  • Primer removal.
  • Plasmid concentration.

Performance Characteristics - Vivacon® 500 for DNA

Sample size
(bp)
Time to concentrate
up to 30x [min.]
at 20°C
Concentrate
recovery %
g-force
(xg)
2,000 MWCO 10 60 min 93 % 7,500
10,000 MWCO 30 25 min 94 % 7,500
30,000 MWCO 50 18 min 88 % 5,000
50,000 MWCO 300 18 min 91 % 3,000
100,000 MWCO 600 10 min 87 % 3,000
125,000 MWCO 650 12 min 85 % 2,000
125,000 MWCO 900 9 min 94 % 3,000

Start volume 0.5 ml, sample concentration 50 ng/ml

Performance Characteristics - Vivacon® 500 for proteins

Sample Time to concentrate
up to 30x [min.]
at 20°C
Concentrate
recovery %
g-force
(xg)
2,000 MWCO 0.25 mg/ml cytochrome c 30 min 95 % 14,000
10,000 MWCO 0.25 mg/ml cytochrome c 15 min 92 % 14,000
30,000 MWCO 1.0 mg/ml BSA 10 min 95 % 14,000
50,000 MWCO 1.0 mg/ml BSA 10 min 92 % 14,000
100,000 MWCO 1.0 mg/ml bovine lgG 11 min 90 % 8,000
100,000 MWCO 0.02 mg/ml porcine hematin 10 min * 88 % 2,500
125,000 MWCO 1.0 mg/ml bovine lgG 10 min 81 % 8,000
125,000 MWCO 0.02 mg/ml porcine hematin 10 min * 11 % 2,500

Start volume 0.5 ml, sample and concentration of protein as specified in table

* Note that the 10 minute spin for the hematin samples gave a concentration factor of 6x for the 100,000 MWCO and 75x for the 125,000 MWCO

Conversion Table for Hydrosart® MWCO to Nucleotide Cut-off

Membrane MWCO Double-Stranded
Nucleotide Cut-off
(bp)
Hydrosart® 2 kDa > 10
Hydrosart® 10 kDa > 30
Hydrosart® 30 kDa > 50
Hydrosart® 50 kDa > 300
Hydrosart® 100 kDa > 600
Cellulose Triacetate 125 kDa > 650

Features & Benefits

Feature Benefit
Low binding material High recoveries of low sample recovery
Re-spin possibility Complete and highly reproducible sample recovery

Tech Specs

Concentrator Capacity
Fixed Angle Rotor 0.5 ml
Dimensions
Total Length 45 mm (concentration)
Total Length 47.5 mm (backspin)
Width 12.4 mm
Active Membrane Area 0.32 cm²
Hold-up volume membrane and support < 5 µl
Dead stop volume 5 µl (40° rotor)
Materials of Construction
Body Polycarbonate
Filtrate vessel Polypropylene
Back spin vial Polypropylene
O Ring Silicone
Membrane (ex 125,000 MWCO) Hydrosart®
Membrane (125,000 MWCO) Cellulose Triacetate
Equipment Required - Centrifuge
Rotor type Fixed angle
Minimum rotor angle 40°
Rotor cavity To fit 1.5 | 2.2 ml (11 mm) conical bottom tubes
Maximum speed 5,000 g (May be increased in some cases. See manual for details).
Also note that DNA recovery may improve at lower g forces.

Ordering Information

Description Prod. No. Price
2,000 MWCO, Hydrosart Membrane, 25 per pack VN01H91 $134.00
Add to Cart
2,000 MWCO, Hydrosart Membrane, 100 per pack VN01H92 $440.00
Add to Cart
10,000 MWCO, Hydrosart Membrane, 25 per pack VN01H01 $134.00
Add to Cart
10,000 MWCO, Hydrosart Membrane, 100 per pack VN01H02 $440.00
Add to Cart
30,000 MWCO, Hydrosart Membrane, 25 per pack VN01H21 $134.00
Add to Cart
30,000 MWCO, Hydrosart Membrane, 100 per pack VN01H22 $440.00
Add to Cart
30,000 MWCO, Hydrosart Membrane, 500 per pack
 
For larger quantities - please contact us for a quotation.
VN01H23 $1,850.00
Add to Cart
50,000 MWCO, Hydrosart Membrane, 25 per pack VN01H31 $134.00
Add to Cart
50,000 MWCO, Hydrosart Membrane, 100 per pack VN01H32 $440.00
Add to Cart
50,000 MWCO, Hydrosart Membrane, 500 per pack
 
For larger quantities - please contact us for a quotation.
VN01H33 $1,850.00
Add to Cart
100,000 MWCO, Hydrosart Membrane, 25 per pack VN01H41 $134.00
Add to Cart
100,000 MWCO, Hydrosart Membrane, 100 per pack VN01H42 $440.00
Add to Cart
100,000 MWCO, Hydrosart Membrane, 500 per pack
 
For larger quantities - please contact us for a quotation.
VN01H43 $1,850.00
Add to Cart
125,000 MWCO, Cellulose Triacetate Membrane, 25 per pack VN01H81 $134.00
Add to Cart
125,000 MWCO, Cellulose Triacetate Membrane, 100 per pack VN01H82 $440.00
Add to Cart
125,000 MWCO, Cellulose Triacetate Membrane, 500 per pack
 
For larger quantities - please contact us for a quotation.
VN01H83 $1,850.00
Add to Cart

Accessories Ordering Information


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Description Prod. No. Price
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