Vivacon® 500
Reproducible DNA sample desalting and concentration
Vivacon® 500 centrifugal concentrators offer the optimal solution for DNA concentration and buffer exchange applications. For optimal performance with very dilute samples, e. g. forensic samples, Vivacon® 500 is equipped with the patented regenerated cellulose membrane Hydrosart®. High recoveries and excellent reproducibilities are paired with convenience offered by volume graduation and molecular weight cut-off printed on individual devices.
Vivacon® 500 can be used as well for concentration and buffer exchange of protein samples.
Vivacon® 500 Applications
- DNA rebuffering of organic extraction samples before sequencing reaction
- Dye removal after sequencing reaction
- Primer removal
- Plasmid concentration
- Protein concentration
- Protein rebuffering
For larger volumes up to 2 ml Vivacon® 2 with identical features is available.
Applications
- DNA rebuffering of organic extraction samples before sequencing reaction.
- Dye removal after sequencing reaction.
- Primer removal.
- Plasmid concentration.
Performance Characteristics - Vivacon® 500 for DNA
|
Sample size (bp) |
Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) |
|
| 2,000 MWCO | 10 | 60 min | 93 % | 7,500 |
| 10,000 MWCO | 30 | 25 min | 94 % | 7,500 |
| 30,000 MWCO | 50 | 18 min | 88 % | 5,000 |
| 50,000 MWCO | 300 | 18 min | 91 % | 3,000 |
| 100,000 MWCO | 600 | 10 min | 87 % | 3,000 |
| 125,000 MWCO | 650 | 12 min | 85 % | 2,000 |
| 125,000 MWCO | 900 | 9 min | 94 % | 3,000 |
Start volume 0.5 ml, sample concentration 50 ng/ml
Performance Characteristics - Vivacon® 500 for proteins
| Sample |
Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) |
|
| 2,000 MWCO | 0.25 mg/ml cytochrome c | 30 min | 95 % | 14,000 |
| 10,000 MWCO | 0.25 mg/ml cytochrome c | 15 min | 92 % | 14,000 |
| 30,000 MWCO | 1.0 mg/ml BSA | 10 min | 95 % | 14,000 |
| 50,000 MWCO | 1.0 mg/ml BSA | 10 min | 92 % | 14,000 |
| 100,000 MWCO | 1.0 mg/ml bovine lgG | 11 min | 90 % | 8,000 |
| 100,000 MWCO | 0.02 mg/ml porcine hematin | 10 min * | 88 % | 2,500 |
| 125,000 MWCO | 1.0 mg/ml bovine lgG | 10 min | 81 % | 8,000 |
| 125,000 MWCO | 0.02 mg/ml porcine hematin | 10 min * | 11 % | 2,500 |
Start volume 0.5 ml, sample and concentration of protein as specified in table
* Note that the 10 minute spin for the hematin samples gave a concentration factor of 6x for the 100,000 MWCO and 75x for the 125,000 MWCO
Conversion Table for Hydrosart® MWCO to Nucleotide Cut-off
| Membrane | MWCO |
Double-Stranded Nucleotide Cut-off (bp) |
| Hydrosart® | 2 kDa | > 10 |
| Hydrosart® | 10 kDa | > 30 |
| Hydrosart® | 30 kDa | > 50 |
| Hydrosart® | 50 kDa | > 300 |
| Hydrosart® | 100 kDa | > 600 |
| Cellulose Triacetate | 125 kDa | > 650 |
Features & Benefits
| Feature | Benefit | Low binding material | High recoveries of low sample recovery |
| Re-spin possibility | Complete and highly reproducible sample recovery |
Tech Specs
| Concentrator Capacity | |
| Fixed Angle Rotor | 0.5 ml |
| Dimensions | |
| Total Length | 45 mm (concentration) |
| Total Length | 47.5 mm (backspin) |
| Width | 12.4 mm |
| Active Membrane Area | 0.32 cm² |
| Hold-up volume membrane and support | < 5 µl |
| Dead stop volume | 5 µl (40° rotor) |
| Materials of Construction | |
| Body | Polycarbonate |
| Filtrate vessel | Polypropylene |
| Back spin vial | Polypropylene |
| O Ring | Silicone |
| Membrane (ex 125,000 MWCO) | Hydrosart® |
| Membrane (125,000 MWCO) | Cellulose Triacetate |
| Equipment Required - Centrifuge | |
| Rotor type | Fixed angle |
| Minimum rotor angle | 40° |
| Rotor cavity | To fit 1.5 | 2.2 ml (11 mm) conical bottom tubes |
| Maximum speed |
5,000 g (May be increased in some cases. See manual for details). Also note that DNA recovery may improve at lower g forces. |
Ordering Information
| Description | Prod. No. | Price | |
| 2,000 MWCO, Hydrosart Membrane, 25 per pack | VN01H91 |
$130.00 |
Add to Cart |
| 2,000 MWCO, Hydrosart Membrane, 100 per pack | VN01H92 |
$430.00 |
Add to Cart |
| 10,000 MWCO, Hydrosart Membrane, 25 per pack | VN01H01 |
$130.00 |
Add to Cart |
| 10,000 MWCO, Hydrosart Membrane, 100 per pack | VN01H02 |
$430.00 |
Add to Cart |
| 30,000 MWCO, Hydrosart Membrane, 25 per pack | VN01H21 |
$130.00 |
Add to Cart |
| 30,000 MWCO, Hydrosart Membrane, 100 per pack | VN01H22 |
$430.00 |
Add to Cart |
|
30,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN01H23 |
$1,805.00 |
Add to Cart |
| 50,000 MWCO, Hydrosart Membrane, 25 per pack | VN01H31 |
$130.00 |
Add to Cart |
| 50,000 MWCO, Hydrosart Membrane, 100 per pack | VN01H32 |
$430.00 |
Add to Cart |
|
50,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN01H33 |
$1,805.00 |
Add to Cart |
| 100,000 MWCO, Hydrosart Membrane, 25 per pack | VN01H41 |
$130.00 |
Add to Cart |
| 100,000 MWCO, Hydrosart Membrane, 100 per pack | VN01H42 |
$430.00 |
Add to Cart |
|
100,000 MWCO, Hydrosart Membrane, 500 per pack
For larger quantities - please contact us for a quotation. |
VN01H43 |
$1,805.00 |
Add to Cart |
| 125,000 MWCO, Cellulose Triacetate Membrane, 25 per pack | VN01H81 |
$130.00 |
Add to Cart |
| 125,000 MWCO, Cellulose Triacetate Membrane, 100 per pack | VN01H82 |
$430.00 |
Add to Cart |
|
125,000 MWCO, Cellulose Triacetate Membrane, 500 per pack For larger quantities - please contact us for a quotation. |
VN01H83 |
$1,805.00 |
Add to Cart |
How to Order
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