Try our Urine Protein Concentration Calculator

Vivaflow® 200 TFF Cassettes

Up to 5 L starting volume
Concentrate up to 125x

Vivaflow® 200 is the original plug-and-play tangential flow filtration (TFF) cassette, for ultrafiltration and diafiltration of intermediate sample volumes. It has been designed for ease of use in R&D labs for proteins, nanoparticles, nucleic acids and virus samples. Vivaflow® 200 features a wide assortment of membrane materials and MWCOs. It offers a budget-friendly solution that does not need expensive cassette holders and can be cleaned in place (CIP) to allow multiple reuses.

Data Sheet

Manual

Cell culture and process fluid clarification
Protein, nucleic acid and virus concentration
Nanoparticle separation and enrichment
Sample volume reduction
Diafiltration for protein solubilization and refolding
Buffer Exchange for chromatography sample preparation
Therapeutic formulation development
Diafiltration for protein-ligand binding studies

Vivaflow® 200

	Time to concentrate 20x (min.) at 3 bar inlet, 20°C
	Start volume	Average flux	Solute Recovery
	1 L	mL \| min	Direct	25 mL rinse
Lysozyme 0.25 mg/mL (14 kDa MW)
2 kDa Hydrosart	160 min.	6	97%	> 99%
3 kDa PES	180 min.	5	97%	> 99%
BSA 1 mg/mL (66 kDa MW)
5 kDa PES	29 min.	33	98%	> 99%
5 kDa Hydrosart	70 min.	14	98%	> 99%
10 kDa PES	23 min.	41	96%	> 99%
10 kDa Hydrosart	35 min.	27	98%	> 99%
30 kDa PES	25 min.	38	96%	99%
30 kDa Hydrosart	20 min.	48	96%	> 99%
50 kDa PES	22 min.	43	96%	98%
γ Globulins 1 mg/mL (160 kDa MW)
100 kDa PES	54 min.	18	96%	99%
100 kDa Hydrosart	45 min.	21	96%	99%
Yeast 1 mg/mL (S. cerevisiae)
0.2 um PES	11 min.	86	92%	98%

Dimensions
Length x Height x Width	126 x 138 x 38 mm
Channel Width x Height	10 x 0.4 mm
Active membrane surface	200 cm²
Min. recirculation volume	<20 mL
Non recoverable hold-up	<1.0 mL
Materials of Construction
Housing	Acrylic
Flow channel	Acrylic
Membrane support	Polyethylene
Gasket	Silicone
Fittings	Polypropylene or Polyamide
Tubing	Polyvinyl chloride
Pressure Indicator	Polypropylene, Stainless Steel, Polyoxymethylene, Silicone
Operating Conditions
Throughput per Cassette (rec.)	2500 mL
Flow rate (rec.)	200-400 mL / min
Two cassettes in parallel	500-900 mL / min
Pressure, maximum
At feed port	4 bar
At retentate port	3.5 bar
Feed tube run time, maximum	6 hours
Operating temperature	4-60°C

Refer to Manual in Downloads section

Description	Prod. #	Price	Buy
Vivaflow 200, 0.2μm PES, 1pc SKU: VF20P7 Price: $655.00	VF20P7	$655.00	Add to Cart
Vivaflow 200, 100kDa HY, 1pc SKU: VF20H4 Price: $655.00	VF20H4	$655.00	Add to Cart
Vivaflow 200, 100kDa PES, 1pc SKU: VF20P4 Price: $655.00	VF20P4	$655.00	Add to Cart
Vivaflow 200, 10kDa HY, 1pc SKU: VF20H0 Price: $655.00	VF20H0	$655.00	Add to Cart
Vivaflow 200, 10kDa PES, 1pc SKU: VF20P0 Price: $655.00	VF20P0	$655.00	Add to Cart
Vivaflow 200, 2kDa HY, 1pc SKU: VF20H9 Price: $655.00	VF20H9	$655.00	Add to Cart
Vivaflow 200, 30kDa HY, 1pc SKU: VF20H2 Price: $655.00	VF20H2	$655.00	Add to Cart
Vivaflow 200, 30kDa PES, 1pc SKU: VF20P2 Price: $655.00	VF20P2	$655.00	Add to Cart
Vivaflow 200, 3kDa PES, 1pc SKU: VF20P9 Price: $655.00	VF20P9	$655.00	Add to Cart
Vivaflow 200, 50kDa PES, 1pc SKU: VF20P3 Price: $655.00	VF20P3	$655.00	Add to Cart
Vivaflow 200, 5kDa HY, 1pc SKU: VF20H1 Price: $655.00	VF20H1	$655.00	Add to Cart
Vivaflow 200, 5kDa PES, 1pc SKU: VF20P1 Price: $655.00	VF20P1	$655.00	Add to Cart

Accessories

Description	Prod. #	Price	Buy
Luer Fittings, Female-HB, Size 15, 10pc SKU: VFA036 Price: $13.00	VFA036	$13.00	Add to Cart
Luer Fittings, Female-HB, Size 16, 10pc SKU: VFA032 Price: $13.00	VFA032	$13.00	Add to Cart
Peristaltic Pump Head, 1.6mm WT SKU: VF-APH0001-1 Price: $717.00	VF-APH0001-1	$717.00	Add to Cart
Peristaltic Pump Head, 2.4mm WT SKU: VF-APH0002-1 Price: $717.00	VF-APH0002-1	$717.00	Add to Cart
Peristaltic Pump, 110-240V SKU: VF-APD0001-1 Price: $4,313.00	VF-APD0001-1	$4,313.00	Add to Cart
Pressure Indicator, 1-3bar, 1pc SKU: VFA020 Price: $56.00	VFA020	$56.00	Add to Cart
Pump Tubing, PVC, Size 15, w.Luer, 3m SKU: VFA003 Price: $87.00	VFA003	$87.00	Add to Cart
Sample and DF Reservoir, 500mL SKU: VFA006 Price: $251.00	VFA006	$251.00	Add to Cart
Series Interconnectors, Size 16, 6pc SKU: VFA031 Price: $28.00	VFA031	$28.00	Add to Cart
Tubing, Diafiltration\|Purification, 1pc SKU: VF-ATD0001-1 Price: $23.00	VF-ATD0001-1	$23.00	Add to Cart
Tubing, Vivaflow 200cm² Parallel, 1pc SKU: VF-ATP0012-1 Price: $102.00	VF-ATP0012-1	$102.00	Add to Cart
Tubing, Vivaflow 50cm² Parallel, 1pc SKU: VF-ATP0011-1 Price: $102.00	VF-ATP0011-1	$102.00	Add to Cart
Tubing, Vivaflow Individual, 1pc SKU: VF-ATI0011-1 Price: $84.00	VF-ATI0011-1	$84.00	Add to Cart
Vivaflow Reservoir, 0.5L, 1pc SKU: VF-ARV0500-1 Price: $229.00	VF-ARV0500-1	$229.00	Add to Cart
Vivaflow Restrictors, 0.4/0.8mm, 1pc/ea SKU: VF-AC-0002-V Price: $12.00	VF-AC-0002-V	$12.00	Add to Cart
Vivaflow SU Stand, 1pc SKU: VF-AST0001-1 Price: $266.00	VF-AST0001-1	$266.00	Add to Cart