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Vivaproducts - Protein Purification and Separation Technologies

Vivaflow® 50R TFF Cassettes

Vivaflow® 50R TFF Cassettes

Up to 1 L starting volume
Concentrate up to 50x

Vivaflow® 50R is the original plug-and-play tangential flow filtration (TFF) cassette, for ultrafiltration and diafiltration of intermediate sample volumes. It has been designed for ease of use in R&D labs for proteins, nanoparticles, nucleic acids and virus samples. Vivaflow® 50R features a wide assortment of membrane materials and MWCOs. It offers a budget-friendly solution that does not need expensive cassette holders and can be cleaned in place (CIP) to allow multiple reuses.

  • Cell culture and process fluid clarification
  • Protein, nucleic acid and virus concentration
  • Nanoparticle separation and enrichment
  • Sample volume reduction
  • Diafiltration for protein solubilization and refolding
  • Buffer Exchange for chromatography sample preparation
  • Therapeutic formulation development
  • Diafiltration for protein-ligand binding studies

Vivaflow® 50R

Time to concentrate 20x (min.) at 3 bar inlet | 2.5 bar outlet pressure, 20°C
Start Volume Average flux Solute Recovery
250 mL mL | min Direct 25 mL rinse
Lysozyme 0.25 mg/mL (14 kDa MW)
5 kDa Hydrosart 70 min. 3.4 96% 98%
10 kDa Hydrosart 23 min. 10.3 94% 96%
BSA 1 mg/mL (66 kDa MW)
10 kDa Hydrosart 24 min. 9.9 98% > 99%
30 kDa Hydrosart 15 min. 15.8 97% > 99%
γ Globulins 1 mg/mL (150 kDa MW)
100 kDa Hydrosart 46 min. 5.2 97% > 99%
Start volume 1 L (one Vivaflow 50R) 10 kDa Hydrosart
BSA 1 mg/mL 95 min. 10.0 98% > 99%
Start volume 1 L (two Vivaflow 50R in parallel) 10 kDa Hydrosart
BSA 1 mg/mL 48 min. 19.8 98% > 99%
Dimensions
Length x Height x Width 100 x 100 x 24 mm
Channel Width x Height 7.5 x 0.4 mm
Active membrane surface 50 cm2
Min. recirculation volume 10 mL
Non recoverable hold-up <0.5 mL
Materials of Construction
Housing Acrylic
Flow channel Acrylic
Membrane support Polyethylene
Gasket Silicone
Fittings Polypropylene or Polyamide
Tubing Polyvinyl chloride
Pressure Indicator Polypropylene, Stainless Steel, Polyoxymethylene, Silicone
Operating Conditions
Throughput per Cassette (rec.) 500 mL
Flow rate (rec.) 200-400 mL / min
Pressure, maximum
At feed port 4 bar
At retentate port 3.5 bar
Feed tube run time, maximum 6 hours
Operating temperature 4-60°C
Refer to Manual in Downloads section
Description Buy
Vivaflow 50R, 100kDa HY, 1pc
SKU: VF05H4
Price: $355.00
Vivaflow 50R, 10kDa HY, 1pc
SKU: VF05H0
Price: $355.00
Vivaflow 50R, 30kDa HY, 1pc
SKU: VF05H2
Price: $355.00
Vivaflow 50R, 5kDa HY, 1pc
SKU: VF05H1
Price: $355.00

Accessories

Description Buy
Luer Fittings, Female-HB, Size 15, 10pc
SKU: VFA036
Price: $13.00
Luer Fittings, Female-HB, Size 16, 10pc
SKU: VFA032
Price: $13.00
Peristaltic Pump Head, 1.6mm WT
SKU: VF-APH0001-1
Price: $717.00
Peristaltic Pump Head, 2.4mm WT
SKU: VF-APH0002-1
Price: $717.00
Peristaltic Pump, 110-240V
SKU: VF-APD0001-1
Price: $4,313.00
Pressure Indicator, 1-3bar, 1pc
SKU: VFA020
Price: $56.00
Pump Tubing, PVC, Size 15, w.Luer, 3m
SKU: VFA003
Price: $87.00
Sample and DF Reservoir, 500mL
SKU: VFA006
Price: $251.00
Series Interconnectors, Size 16, 6pc
SKU: VFA031
Price: $28.00
Tubing, Diafiltration|Purification, 1pc
SKU: VF-ATD0001-1
Price: $23.00
Tubing, Vivaflow 200cm² Parallel, 1pc
SKU: VF-ATP0012-1
Price: $102.00
Tubing, Vivaflow 50cm² Parallel, 1pc
SKU: VF-ATP0011-1
Price: $102.00
Tubing, Vivaflow Individual, 1pc
SKU: VF-ATI0011-1
Price: $84.00
Vivaflow Reservoir, 0.5L, 1pc
SKU: VF-ARV0500-1
Price: $229.00
Vivaflow Restrictors, 0.4/0.8mm, 1pc/ea
SKU: VF-AC-0002-V
Price: $12.00
Vivaflow SU Stand, 1pc
SKU: VF-AST0001-1
Price: $266.00

How to Order

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