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Vivaproducts - Protein Purification and Separation Technologies

Vivapure® Ion Exchange Spin Columns

Vivapure® Ion Exchange Spin Columns

0.24 to 2.7 mL bed volume

The Vivapure® ion exchange spin columns use a centrifugal format with a Sartorius membrane support for ion exchange chromatography. The ligands are covalently bound to the internal microporous surfaces providing very high binding capacities. The membrane pores also allow rapid mass transfer and faster sample flow rates. These products save valuable time by eliminating the need to pack columns and are also designed for multiple reuses.

  • Cell culture and process fluid clarification
  • Protein, nucleic acid and virus concentration
  • Nanoparticle separation and enrichment
  • Sample volume reduction
  • Diafiltration for protein solubilization and refolding
  • Buffer Exchange for chromatography sample preparation
  • Therapeutic formulation development
  • Diafiltration for protein-ligand binding studies
Membrane Adsorber
Vivapure S Vivapure Q Vivapure D
Nominal pore size 3-5 µm 3-5 µm 3-5 µm
Thickness 230-320 µm 230-320 µm 230-320 µm
Ligand density 145-218 µEq/mL 145-218 µEq/mL 145-218 µEq/mL
Ligand Type Strong acidic cation Strong basic anion Weak basic anion
Functional Group Sulphonic acid Quarternary ammonium Diethylamine
Working pH 2-12 2-12 4-10
Approximate pKa 1 11 9.5
Capacities & Dimensions
Vivapure S/Q/D-Mini Vivapure S/Q/D-Maxi
Sample volume, fixed angle <0.4 mL <10.5 mL
Sample volume, swing out N/A <19 mL
Binding capacity * 4 mg 60-80 mg
Membrane area 7.48 cm2 84.4 cm2
Bed Volume 0.24 mL 2.7 mL
Materials of Construction
Vivapure S/Q/D-Mini Vivapure S/Q/D-Maxi
Housing Polypropylene Polypropylene
Membrane support Stabilized regenerated cellulose Stabilized regenerated cellulose

* Binding capacities established using 1 mg/mL BSA in 25 mM Tris-HCl (pH 8) for Vivapure® Q and D devices, or 1 mg/mL cytochrome c in 25 mM sodium acetate buffer (pH 5.5) for Vivapure® S devices. Actual capacities depend on the target molecule and selected buffer conditions.

Refer to Manual in Downloads section
Description Buy
Vivapure D Maxi H, 8pc
SKU: VS-IX20DH08
Price: $526.00
Vivapure D Mini H, 24pc
SKU: VS-IX01DH24
Price: $278.00
Vivapure Q Maxi H, 8pc
SKU: VS-IX20QH08
Price: $526.00
Vivapure Q Mini H, 24pc
SKU: VS-IX01QH24
Price: $278.00
Vivapure S Maxi H, 8pc
SKU: VS-IX20SH08
Price: $526.00
Vivapure S Mini H, 24pc
SKU: VS-IX01SH24
Price: $278.00

Accessories

Description Buy
Peristaltic Pump Head, 1.6mm WT
SKU: VF-APH0001-1
Price: $717.00
Peristaltic Pump Head, 2.4mm WT
SKU: VF-APH0002-1
Price: $717.00
Peristaltic Pump, 110-240V
SKU: VF-APD0001-1
Price: $4,313.00
Sartobind Lab LC System Adapter Kit
SKU: SBLAAU01-1
Price: $25.00
Tubing, Diafiltration|Purification, 1pc
SKU: VF-ATD0001-1
Price: $23.00
Vivapure Adenopack 100, Pump Tubing Set
SKU: VS-AVPA001
Price: $35.00

How to Order

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