Purify up to 1000 mL sample volumes
The Vivapure® Adenopack and Lentiselect products are complete kits for efficient purification of adenovirus type 5 and VSV-G pseudotyped lentivirus. They feature the same membrane adsorber technology as other Vivapure products providing high binding capacities and faster sample flow rates. These kits come complete with filters and buffers and provide faster separations not possible with cesium chloride density gradients or ultracentrifugation methods.
Manual - Vivapure® Adenopack™ 20
Manual - Vivapure® Adenopack™ 20 RT
Manual - Vivapure® Adenopack™ 100
Manual - Vivapure® Adenopack™ 100 RT
Manual - Vivapure® Adenopack™ 500
Manual - Vivapure® Adenopack™ 500 RT
Manual - Vivapure® Lentiselect 40
Manual - Vivapure® Lentiselect 500
Manual - Vivapure® Lentiselect 1000
Lab Chromatography Brochure
| Purification of Ad5-GFP Constructs | |||||
|---|---|---|---|---|---|
| Purification Method | Sample Volume | Process Time | Eluate | Recovery† | Viral Particles |
| Adenopack 20 | 20 mL | 1 hr | 1 mL | 65 – 70% | 1 × 1011-12 |
| Adenopack 100 | 60 mL | 1 – 2 hr | 1 mL | 65% | 1 – 3 × 1012 |
| Adenopack 100 | 200 mL | 2 hr | 1 mL | 80% | 1 × 1013 |
| Adenopack 500 | 500 mL | 2 hr | 1 mL | 80% | 1 – 3 × 1013 |
| CsCl density gradient | 500 mL | 24 – 48 hr | 1 – 2 mL* | 60 – 70% | 1 × 1011_12 |
* After dialysis
† Before buffer exchange
| Purification of VSV-G Pseudotyped Lentivirus Constructs | |||||
|---|---|---|---|---|---|
| Kit | Sample Volume | Process Time | Eluate | Recovery | Viral Particles |
| Lentiselect 40 | 40 mL | 45 min | 0.2 mL* | 50% | 8 × 108 |
| Lentiselect 500 | 500 mL | 3 hr | 1 mL* | 35% | 2-5 × 109 |
| Lentiselect 1000 | 1,000 mL | 6 hr | 2 mL* | 35% | 1 × 1010 |
| Ultracentrifugation | 500 mL | 10 - 11 hr | 0.5 mL | 25% | 3 × 109 |
* After desalting | buffer exchange
| Vivapure® Adenopack Kits | |||
| Adenopack 20 | Adenopack 100 | Adenopack 500 | |
| Number of purifications | 6 × 20 mL | 2 × 20 - 60 mL or 1 × 200 mL |
1 × 500 mL |
| Typical virus titre | 1 × 1011 - 1 × 1012 VP/mL | 1 × 1013 VP/mL | 3 × 1013 VP/mL |
| Viral particle to plaque forming unit ratio (VP/PFU) | 50 - 100 | 20 - 50 | 20 - 50 |
| Typical processing time | 1 hr | 2 hr | 2 hr |
| Endotoxin level | < 0.025 EU/mL | < 0.025 EU/mL | < 0.025 EU/mL |
| Vivapure® Lentiselect Kits | |||
| Lentiselect 40 | Lentiselect 100 | Lentiselect 500 | |
| Number of purifications | 4 × 40 mL | 1 × 500 mL | 1 × 1,000 mL |
| Typical number of infective particles | 3 x 109 IP/mL | 2-5 x 109 IP/mL | 4-5 x 109 IP/mL |
| Final sample volume | 0.2 mL | 1 mL | 2 mL |
| Viral particle to plaque forming unit ratio (VP/PFU) | 5 - 15 | 20 - 50 | 20 - 50 |
| Typical processing time | 45 min | 3 hr | 6 hr |
| Endotoxin level | < 0.025 EU/mL | < 0.025 EU/mL | < 0.025 EU/mL |
| Refer to Manual in Downloads section |
| Description | Prod. # | Price | Buy |
|---|---|---|---|
|
Vivapure Adenopack 100 RT
SKU: VS-AVPQ102
Price: $598.00 |
Add to Cart | ||
|
Vivapure Adenopack 100, with Benzonase
SKU: VS-AVPQ101
Price: $806.00 |
Add to Cart | ||
|
Vivapure Adenopack 20 RT
SKU: VS-AVPQ022
Price: $335.00 |
Add to Cart | ||
|
Vivapure Adenopack 20, with Benzonase
SKU: VS-AVPQ020
Price: $449.00 |
Add to Cart | ||
|
Vivapure Adenopack 500 RT
SKU: VS-AVPQ502
Price: $1,957.00 |
Add to Cart | ||
|
Vivapure Adenopack 500, with Benzonase
SKU: VS-AVPQ501
Price: $1,762.00 |
Add to Cart | ||
|
Vivapure Lentiselect 1000
SKU: VS-LVPQ1000
Price: $2,843.00 |
Add to Cart | ||
|
Vivapure Lentiselect 40
SKU: VS-LVPQ040
Price: $867.00 |
Add to Cart | ||
|
Vivapure Lentiselect 500
SKU: VS-LVPQ500
Price: $1,708.00 |
Add to Cart |
| Description | Prod. # | Price | Buy |
|---|---|---|---|
|
Peristaltic Pump Head, 1.6mm WT
SKU: VF-APH0001-1
Price: $717.00 |
Add to Cart | ||
|
Peristaltic Pump Head, 2.4mm WT
SKU: VF-APH0002-1
Price: $717.00 |
Add to Cart | ||
|
Peristaltic Pump, 110-240V
SKU: VF-APD0001-1
Price: $4,313.00 |
Add to Cart | ||
|
Sartobind Lab LC System Adapter Kit
SKU: SBLAAU01-1
Price: $25.00 |
Add to Cart | ||
|
Tubing, Diafiltration|Purification, 1pc
SKU: VF-ATD0001-1
Price: $23.00 |
Add to Cart | ||
|
Vivapure Adenopack 100, Pump Tubing Set
SKU: VS-AVPA001
Price: $35.00 |
Add to Cart |
0.4 to 2.8 mL bed volume
Perform rapid ion exchange or affinity chromatography in a convenient syringe filter format. Available in three sizes and designed for multiple reuses.
Clarify up to 0.5 mL sample volumes
Rapidly filter and clarify biological samples up to 0.5 mL in volume in a convenient 0.8 µm filter spin column format.
0.24 to 2.7 mL bed volume
Perform rapid ion exchange chromatography in a convenient spin column filter format. Available in two sizes and designed for multiple reuses.
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