Vivaspin® 15R Centrifugal Concentrator

Vivaspin® 15R Centrifugal Concentrator

Up to 15 ml starting volume
Concentrate up to 400x or more
Vivaspin® 15R is the latest member of the Vivaspin® product family with all the unique features of these concentrators including a patented vertical membrane and a dead stop. Vivaspin® 15R is used for sample volumes up to 15 ml with a modified regenerated cellulose membrane; Hydrosart®. This membrane is ideal where extremely high recovery with very low adsorption is needed, for example in applications such as desalting and concentration of Ig fractions.

  • Ultimate recovery at low adsorption (95-98%)
  • Extremely short concentration time (30x in 15 min.)
  • Convenient application protocol with easy handling
  • Easy scale-up to Vivaflow 200 with Hydrosart® membrane for volumes up to 5 litres
  • Very small hold up volume (< 20 µl)

Rotor Compatibility
Please note:
Vivaspin® 15R (30 mm x 116 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus / Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes.

These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 Beckmann JA 20. If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the Vivaspin® 15, which can be used in either round bottom or conical centrifuge tubes.

Applications

  • Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis.
  • Concentrate spinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis.
  • Protein removal from serum & cell / tissue lysates.
  • Exchange buffers for proteomic peptide samples.
  • Prepare samples for mass spectrometry.

Performance Characteristics

Time to concentrate up to 30x [min.]
at 20°C and solute recovery %
Rotor Swing bucket 25 ° Fixed angle
Centrifugal force 3,000 x g 6,000 x g
Start volume 15 ml 12.5 ml
Min. Rec. Min. Rec.
Aprotonin 0.1 mg / ml* (6,500 MW)
5,000 MWCO HY 47 95% 45 95%
Cytochrome c 0.25 mg / ml* (12,400 MW)
5,000 MWCO HY 45 96% 45 96%
10,000 MWCO HY 25 94% 18 94%
α-chymotrypsin 0.25 mg / ml* (25,000 MW)
5,000 MWCO HY 50 98% 45 98%
10,000 MWCO HY 25 98% 18 98%
Ovalbumin 1.0 mg / ml* (45,000 MW)
10,000 MWCO HY 20 98% 14 98%
30,000 MWCO HY 15 94% 12 94%
BSA 1.0 mg / ml* (66,000 MW)
30,000 MWCO HY 18 98% 15 98%
IgG 0.10 mg / ml* in DMEM (160,000 MW)
30,000 MWCO HY 30 98% 25 96%

* proteins other than IgG made up in 50 mM potassium phosphate, 150 mM sodium chloride, pH 7.4

Tech Specs

Concentrator Capacity
Swing bucket rotor 15 ml
Fixed angle rotor 12.5 ml
Dimensions
Total Length 116 mm
Width 30 mm
Active Membrane Area 3.9 cm²
Hold-up volume membrane < 20 µl
Dead stop volume 30 µl
Materials of Construction
Body Polycarbonate
Filtrate Vessel Polycarbonate
Concentrator Cap Polypropylene
Membrane Hydrosart®

Equipment Required

Centrifuge
Rotor Type Swing bucket Fixed angle
Minimum rotor angle - 25°
Rotor Cavity To fit 50 ml (30 mm) conical bottom tubes To fit 50 ml (30 mm) conical bottom tubes
Maximum Speed 3,000 g 6,000 g
Concentrate Recovery
Pipette type Fixed or variable volume Fixed or variable volume
Recommended tip Thin gel loader type Thin gel loader type

Ordering Information

Description Prod. No. Price
2,000 MWCO, Hydrosart Membrane, 12 per pack VS15RH91 $131.00
Add to Cart
2,000 MWCO, Hydrosart Membrane, 48 per pack VS15RH92 $445.00
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5,000 MWCO, Hydrosart Membrane, 12 per pack VS15RH11 $131.00
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5,000 MWCO, Hydrosart Membrane, 48 per pack VS15RH12 $445.00
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10,000 MWCO, Hydrosart Membrane, 12 per pack VS15RH01 $131.00
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10,000 MWCO, Hydrosart Membrane, 48 per pack VS15RH02 $445.00
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30,000 MWCO, Hydrosart Membrane, 12 per pack VS15RH21 $131.00
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30,000 MWCO, Hydrosart Membrane, 48 per pack VS15RH22 $445.00
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20,000 MWCO, Cellulose Triacetate Membrane, 12 per pack VS15RXETO $142.00
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