Vivaspin® 20

Vivaspin® 20

Up to 20 ml starting volume
Concentrate up to 400x
The Vivaspin® 20ml centrifugal concentrators have been developed to offer increased volume flexibility and performance.

Vivaspin® 20 handles up to 20ml in swing bucket centrifuges 14ml in 25° fixed angle rotors accepting 50ml centrifuge tubes. Featuring twin vertical membranes for unparalleled filtration speeds and 100X plus concentrations. Remaining volume is easy to read off the printed scale on the side of the concentrator and the modified dead stop pocket further simplifies direct pipette recovery of the final concentrate.

Process Flexibility
Vivaspin® 20 is available with unique accessories and operating methods that are designed to provide more process flexibility and further time saving.

One Step Desalting
In this procedure following concentration, a diafiltration cup is filled with buffer and then spun one time to achieve 98% salt removal. This compares to the need for two spins to achieve the same result with the traditional refill and re-spin procedure.

The improved performance is due to the constant washing action of the buffer solution in the accessory cup as it replaces solvent and salts as they pass through the ultrafiltration membrane.

Gas Pressure Filtration
When an appropriate centrifuge is unavailable, or for single sample processing, Vivaspin® 20 can be filled with up to 15ml and then pressurised for bench top concentration. For even faster processing, Gas pressure can be combined with centrifugal force. "Pressure-fugation" is particularly suitable for difficult or viscous samples such as serum, or when using a low process temperature which reduces filtration speed, and generally when minimum process time is essential.

Rotor Compatibility
Please note: Vivaspin® 20 (30 mm x 116 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus / Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes.

These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 or Beckmann JA 20.

If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the Vivaspin® 15, which can be used in either round bottom or conical centrifuge tubes.

Applications

  • Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis.
  • Concentrate spinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis.
  • Protein removal from serum & cell / tissue lysates.
  • Exchange buffers for proteomic peptide samples.
  • Prepare samples for mass spectrometry.

Performance Characteristics

  Time to concentrate up to 30x [min.] at 20°C and solute recovery %
Mode Centrifuge Centrifuge Benchtop Pressure Fuge
Type Swing Bucket 25° fixed Angle Pressure Swing Bucket
Pressure/Speed 3,000 g 6,000 g 4 bar 3,000 g
+ 4 bar
Start Volume 20 ml 14 ml 10 ml 10 ml
  Min. Rec. Min. Rec. Min. Rec. Min. Rec.
Cytochrome c 0.25 mg/ml (12,400 MW)
3,000 MWCO PES 110 97% 180 96% 60 96% - -
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO PES 23 99% 29 99% 50 98% 14 98%
10,000 MWCO PES 16 98% 17 98% 32 97% 8 97%
30,000 MWCO PES 13 98% 15 98% 32 97% 8 97%
IgG 0.25 mg/ml (160,000 MW)
30,000 MWCO PES 27 97% 20 95% 46 94% 13 97%
50,000 MWCO PES 27 97% 22 95% 46 93% 13 96%
100,000 MWCO PES 25 91% 20 90% 42 88% 12 94%
Latex beads 0.004 % in DMEM +10 % FCS (0.055 µm)
300,000 MWCO PES 20 99% 35 99% 10 99% - -
Latex beads 0.004 % in DMEM +10 % FCS (0.24 µm)
1,000,000 MWCO PES 4 99% 12 99% 4 99% - -
Yeast 1.0 mg/ml (S. Cerevisiae)
0.2 µm PES 15 95% 5 95% 20 95% 2 95%

Tech Specs

Concentrator Capacity
Swing Bucket Rotor 20 ml
25° Fixed Angle Rotor 14 ml
With Pressure Cap 15 ml
Dimensions
Total Length 116 mm
Width 30 mm
Active Membrane Area 6.0 cm²
Hold up Volume Memb. <20 µl
Dead Stop Volume 50 µl
Carrier Required
Insert Diameter 29 mm
Conical Bottom Tube 50 ml
Maximum Pressure 5 bar
Materials of Construction
Body and Filtrate Vessel Polycarbonate
Concentrator Cap Polypropylene
Membranes Polyethersulfone
Pressure Head Acetal & Aluminum

Equipment Required

Centrifuge
Rotor Type Swing bucket Fixed angle
Minimum rotor angle - 25°
Rotor Cavity To fit 50 ml (30 mm) conical bottom tubes To fit 50 ml (30 mm) conical bottom tubes
Maximum Speed 5,000 g* 8,000 g*
Concentrate Recovery
Pipette type Fixed or variable volume Fixed or variable volume
Recommended tip Thin gel loader type Thin gel loader type

* Please note, devices with membrane MWCO ≥ 100 kDa need to be processed at lower g forces. See data sheet for details.

Vivaspin® 20 Ordering Information

Description Prod. No. Price
3,000 MWCO, PES Membrane, 12 per pack VS2091 $147.00
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3,000 MWCO, PES Membrane, 48 per pack VS2092 $535.00
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5,000 MWCO, PES Membrane, 12 per pack VS2011 $147.00
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5,000 MWCO, PES Membrane, 48 per pack VS2012 $535.00
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10,000 MWCO, PES Membrane, 12 per pack VS2001 $147.00
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10,000 MWCO, PES Membrane, 48 per pack
 
For larger quantities - please contact us for a quotation.
VS2002 $535.00
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30,000 MWCO, PES Membrane, 12 per pack VS2021 $147.00
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30,000 MWCO, PES Membrane, 48 per pack VS2022 $535.00
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50,000 MWCO, PES Membrane, 12 per pack VS2031 $147.00
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50,000 MWCO, PES Membrane, 48 per pack VS2032 $535.00
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100,000 MWCO, PES Membrane, 12 per pack VS2041 $147.00
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100,000 MWCO, PES Membrane, 48 per pack VS2042 $535.00
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300,000 MWCO, PES Membrane, 12 per pack VS2051 $147.00
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300,000 MWCO, PES Membrane, 48 per pack VS2052 $535.00
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1,000,000 MWCO, PES Membrane, 12 per pack VS2061 $147.00
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1,000,000 MWCO, PES Membrane, 48 per pack VS2062 $535.00
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0.2µm, PES Membrane, 12 per pack VS2071 $147.00
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0.2µm, PES Membrane, 48 per pack VS2072 $535.00
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PES Membrane, Start Pack with 2 @ 0.2µm/5K/10K/30K/50K/100K MWCO VS20S1 $147.00
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Vivaspin® 20 Accessories

Description Prod. No. Price
Air pressure controller (APC) complete with pressure gauge, regulator, over-pressure safety valve, female connector, and 1 m extension line (4 mm pneumatic tubing) with male and female connectors and 1 m of 6 mm inlet tubing VCA002 $340.00
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Charge valve for pressure head VCA005 $65.00
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Female connector VCA010 $51.00
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Male connector VCA011 $43.00
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4 mm OD pneumatic tube (3 ml) VCA012 $9.90
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Vivaspin 20 pressure head VCA200 $63.00
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Diafiltration cups, 12 per pack VSA005 $26.00
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