Vivaspin® 4 Turbo Centrifugal Concentrator
Up to 4 ml starting volume
Concentrate up to 100x
Vivaspin® 4 Turbo concentrators are disposable ultrafiltration devices for the concentration of biological samples. Maximum initial sample volumes range up to 4 ml. They can be effectively used in either swing bucket or fixed angle rotors accepting 15 ml centrifuge tubes.
The patented vertical membrane design and thin channel filtration chamber (US 5,647,990) minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions. Macromolecules collect in a 40 µl concentrate pocket which is uniquely angled and molded below the membrane surface, thereby eliminating the risk of filtration to dryness.
The Vivaspin® 4 Turbo is available with the high flux polyethersulfone membrane which is recommended for most solutions.
Applications
- Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis.
- Concentrate spinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis.
- Protein removal from serum & cell / tissue lysates.
- Exchange buffers for proteomic peptide samples.
- Prepare samples for mass spectrometry.
Performance Characteristics
|
Time to concentrate up to 30x [min.] at 20°C |
Concentrate Recovery % |
|
| Rotor | Swing Bucket | |
| Centrifugal force | 4,000 x g | |
| Start volume | 4 ml | |
| Min. | Rec. | |
| BSA 1.0 mg / ml (66,000 MW) | ||
| 10,000 MWCO PES | 10 | 98% |
| 30,000 MWCO PES | 8 | 96% |
| IgG 1.0 mg / ml (160,000 MW) | ||
| 30,000 MWCO PES | 18 | 94% |
| 50,000 MWCO PES | 16 | 93% |
| 100,000 MWCO PES** | 17 | 94% |
** 3,000 x g
Tech Specs
| Concentrator Capacity | |
| Swing Bucket Rotor | 4 ml |
| Fixed Angle Rotor | 4 ml |
| Dimensions | |
| Total Length | 122 mm |
| Width | 14.6 mm |
| Active Membrane Area | 3.2 cm² |
| Hold-up volume of membrane | <10 µl |
| Dead stop volume (Swing Bucket) | 40 µl |
| Dead stop volume (Fixed Angle) | 30 µl |
| Materials of Construction | |
| Body | Styrene butadiene copolymer |
| Filtrate Vessel | Polypropylene |
| Concentrator Cap | Polypropylene |
| Membrane | Polyethersulfone |
Equipment Required
| Centrifuge | ||
| Rotor Type | Swing bucket | Fixed Angle |
| Minimum rotor angle | - | 25° |
| Rotor Cavity | To fit 15 ml (17 mm) conical bottom tubes | To fit 15 ml (17 mm) conical bottom tubes |
| Maximum Speed | 4,000 g* | 7,500 g* |
| Concentrate Recovery | ||
| Pipette type | Fixed or variable volume | Fixed or variable volume |
| Recommended tip | Thin gel loader type | Thin gel loader type |
* Please note, devices with membrane MWCO ≥ 100 kDa need to be processed at lower g forces. See data sheet for details.
Ordering Information
| Description | Prod. No. | Price | |
| 3,000 MWCO, PES membrane, 25 per pack | VS04T91 |
$189.00 |
Add to Cart |
| 3,000 MWCO, PES membrane, 100 per pack | VS04T92 |
$663.00 |
Add to Cart |
| 5,000 MWCO, PES membrane, 25 per pack | VS04T11 |
$189.00 |
Add to Cart |
| 5,000 MWCO, PES membrane, 100 per pack | VS04T12 |
$663.00 |
Add to Cart |
| 10,000 MWCO, PES membrane, 25 per pack | VS04T01 |
$189.00 |
Add to Cart |
| 10,000 MWCO, PES membrane, 100 per pack | VS04T02 |
$663.00 |
Add to Cart |
| 30,000 MWCO, PES membrane, 25 per pack | VS04T21 |
$189.00 |
Add to Cart |
| 30,000 MWCO, PES membrane, 100 per pack | VS04T22 |
$663.00 |
Add to Cart |
| 50,000 MWCO, PES membrane, 25 per pack | VS04T31 |
$189.00 |
Add to Cart |
| 50,000 MWCO, PES membrane, 100 per pack | VS04T32 |
$663.00 |
Add to Cart |
| 100,000 MWCO, PES membrane, 25 per pack | VS04T41 |
$189.00 |
Add to Cart |
| 100,000 MWCO, PES membrane, 100 per pack | VS04T42 |
$663.00 |
Add to Cart |