Vivaspin® 4 Turbo Centrifugal Concentrator

Vivaspin® 4 Turbo Centrifugal Concentrator

Up to 4 ml starting volume
Concentrate up to 100x
Vivaspin® 4 Turbo concentrators are disposable ultrafiltration devices for the concentration of biological samples. Maximum initial sample volumes range up to 4 ml. They can be effectively used in either swing bucket or fixed angle rotors accepting 15 ml centrifuge tubes.

The patented vertical membrane design and thin channel filtration chamber (US 5,647,990) minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions. Macromolecules collect in a 40 µl concentrate pocket which is uniquely angled and molded below the membrane surface, thereby eliminating the risk of filtration to dryness.

The Vivaspin® 4 Turbo is available with the high flux polyethersulfone membrane which is recommended for most solutions.


Applications

  • Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis.
  • Concentrate spinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis.
  • Protein removal from serum & cell / tissue lysates.
  • Exchange buffers for proteomic peptide samples.
  • Prepare samples for mass spectrometry.

Performance Characteristics

Time to concentrate
up to 30x [min.] at 20°C
Concentrate
Recovery %
Rotor Swing Bucket
Centrifugal force 4,000 x g
Start volume 4 ml
Min. Rec.
BSA 1.0 mg / ml (66,000 MW)
10,000 MWCO PES 10 98%
30,000 MWCO PES 8 96%
IgG 1.0 mg / ml (160,000 MW)
30,000 MWCO PES 18 94%
50,000 MWCO PES 16 93%
100,000 MWCO PES** 17 94%

** 3,000 x g

Tech Specs

Concentrator Capacity
Swing Bucket Rotor 4 ml
Fixed Angle Rotor 4 ml
Dimensions
Total Length 122 mm
Width 14.6 mm
Active Membrane Area 3.2 cm²
Hold-up volume of membrane <10 µl
Dead stop volume (Swing Bucket) 40 µl
Dead stop volume (Fixed Angle) 30 µl
Materials of Construction
Body Styrene butadiene copolymer
Filtrate Vessel Polypropylene
Concentrator Cap Polypropylene
Membrane Polyethersulfone

Equipment Required

Centrifuge
Rotor Type Swing bucket Fixed Angle
Minimum rotor angle - 25°
Rotor Cavity To fit 15 ml (17 mm) conical bottom tubes To fit 15 ml (17 mm) conical bottom tubes
Maximum Speed 4,000 g* 7,500 g*
Concentrate Recovery
Pipette type Fixed or variable volume Fixed or variable volume
Recommended tip Thin gel loader type Thin gel loader type

* Please note, devices with membrane MWCO ≥ 100 kDa need to be processed at lower g forces. See data sheet for details.

Ordering Information

Description Prod. No. Price
3,000 MWCO, PES membrane, 12 per pack VS04T91 $152.00
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3,000 MWCO, PES membrane, 48 per pack VS04T92 $535.00
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5,000 MWCO, PES membrane, 12 per pack VS04T11 $152.00
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5,000 MWCO, PES membrane, 48 per pack VS04T12 $535.00
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10,000 MWCO, PES membrane, 12 per pack VS04T01 $152.00
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10,000 MWCO, PES membrane, 48 per pack VS04T02 $535.00
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30,000 MWCO, PES membrane, 12 per pack VS04T21 $152.00
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30,000 MWCO, PES membrane, 48 per pack VS04T22 $535.00
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50,000 MWCO, PES membrane, 12 per pack VS04T31 $152.00
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50,000 MWCO, PES membrane, 48 per pack VS04T32 $535.00
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100,000 MWCO, PES membrane, 12 per pack VS04T41 $152.00
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100,000 MWCO, PES membrane, 48 per pack VS04T42 $535.00
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