Vivaspin® 4 Centrifugal Concentrator

Vivaspin® 4 Centrifugal Concentrator

Up to 4 ml starting volume
Concentrate up to 200x
Vivaspin® 4 ml concentrators are disposable ultrafiltration devices for the concentration of biological samples. Maximum initial sample volumes range up to 4 ml. They can be effectively used in either swing bucket or fixed angle rotors accepting 15 ml centrifuge tubes.

The patented vertical membrane design and thin channel filtration chamber (US 5,647,990) minimises membrane fouling and provides high speed concentrations, even with particle laden solutions.

Vivaspin® 4 is available with the high flux polyethersulfone membrane range which is recommended for most solutions.


Applications

  • Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis.
  • Concentrate spinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis.
  • Protein removal from serum & cell / tissue lysates.
  • Exchange buffers for proteomic peptide samples.
  • Prepare samples for mass spectrometry.

Performance Characteristics

Time to concentrate
up to 30x [min.] at 20°C
Concentrate
Recovery %
Rotor Fixed angle
Centrifugal force 5,000 x g
Start volume 4 ml
Min. Rec.
BSA 1.0 mg / ml (66,000 MW)
5,000 MWCO PES 15 96%
10,000 MWCO PES 10 96%
30,000 MWCO PES 10 95%
IgG 0.25 mg / ml (160,000 MW)
30,000 MWCO PES 10 95%
50,000 MWCO PES 10 95%
100,000 MWCO PES 10 95%

Tech Specs

Concentrator Capacity
Swing Bucket Rotor 4 ml
Fixed Angle Rotor 4 ml
Dimensions
Total Length 122 mm
Width 17 mm
Active Membrane Area 2.0 cm²
Hold-up volume of membrane <10 µl
Dead stop volume 20 µl
Materials of Construction
Body Polycarbonate
Filtrate Vessel Polypropylene
Concentrator Cap Polycarbonate
Membrane Polyethersulfone

Equipment Required

Centrifuge
Rotor Type Swing bucket Fixed Angle
Minimum rotor angle - 25°
Rotor Cavity To fit 15 ml (17 mm) conical bottom tubes To fit 15 ml (17 mm) conical bottom tubes
Maximum Speed 4,000 g 10,000 g*
Concentrate Recovery
Pipette type Fixed or variable volume Fixed or variable volume
Recommended tip Thin gel loader type Thin gel loader type

* Please note, devices with membrane MWCO ≥ 100 kDa need to be processed at lower g forces. See data sheet for details.

Ordering Information

Description Prod. No. Price
10,000 MWCO, PES membrane, 25 per pack VS0403 $150.00
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10,000 MWCO, PES membrane, 100 per pack VS0404 $450.00
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10,000 MWCO, PES membrane, 500 per pack
 
For larger quantities - please contact us for a quotation.
VS0405 $2,080.00
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